Seroatlas · Human Serome Atlas

RPL35

Large ribosomal subunit protein uL29

Also known as: L35, RL35_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P42766
Gene
RPL35
Ensembl
ENSG00000136942
Chromosome
9
Canonical length
123 aa
Protein class
Disease related genes, Human disease related genes, Predicted intracellular proteins, Ribosomal proteins
Subcellular location
Endoplasmic reticulum,Cytosol

OverviewNCBI Gene

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L29P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

123 residues, UniProt reviewed canonical sequence.

>P42766|RPL35
     1  MAKIKARDLR GKKKEELLKQ LDDLKVELSQ LRVAKVTGGA ASKLSKIRVV RKSIARVLTV
    61  INQTQKENLR KFYKGKKYKP LDLRPKKTRA MRRRLNKHEE NLKTKKQQRK ERLYPLRKYA
   121  VKA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPL35 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.46
Highest tissue expression
3,359 nTPM

Expression across tissuesHPA

Tissue

  • pancreas: 3,359 nTPM
  • ovary: 3,000 nTPM
  • skin: 2,958 nTPM
  • bone marrow: 2,570 nTPM
  • skeletal muscle: 2,534 nTPM
  • esophagus: 2,504 nTPM

Single-cell type

  • late spermatids: 9,440 nCPM
  • esophageal suprabasal cells: 7,611 nCPM
  • esophageal basal cells: 6,504 nCPM
  • decidual stromal cells: 5,753 nCPM
  • esophageal apical cells: 5,362 nCPM
  • extravillous trophoblasts: 5,342 nCPM

Immune cell

  • total PBMC: 7,315 nTPM
  • memory B-cell: 4,477 nTPM
  • naive B-cell: 4,122 nTPM
  • plasmacytoid DC: 3,663 nTPM
  • naive CD4 T-cell: 3,230 nTPM
  • MAIT T-cell: 3,109 nTPM

Brain region

  • thalamus: 632 nTPM
  • white matter: 618 nTPM
  • spinal cord: 612 nTPM
  • hypothalamus: 601 nTPM
  • basal ganglia: 568 nTPM
  • medulla oblongata: 568 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RPL35.

Disease | AllUniProt

Conditions RPL35 is implicated in, by any mechanism.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 87 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Diamond-Blackfan anemia 19

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.72
gnomAD pLI
0.55
gnomAD missense Z
1.22
DepMap mean gene effect
-1.51
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Large ribosomal subunit protein uL29
  • Large ribosomal subunit protein uL29, conserved site
  • Large ribosomal subunit protein uL29 superfamily
  • Large ribosomal subunit protein uL29, eukaryota
  • Ribosomal L29 protein

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPL35 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPL35 as an antibody target. Whether an autoantibody or antibody against RPL35 could matter depends on whether native RPL35 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPL35 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPL35 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RPL35. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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