Seroatlas · Human Serome Atlas

RPS16

Small ribosomal subunit protein uS9

Also known as: RS16_HUMAN, S16

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P62249
Gene
RPS16
Ensembl
ENSG00000105193
Chromosome
19
Canonical length
146 aa
Protein class
Plasma proteins, Predicted intracellular proteins, Ribosomal proteins
Subcellular location
Endoplasmic reticulum,Cytosol

OverviewNCBI Gene

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S9P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

146 residues, UniProt reviewed canonical sequence.

>P62249|RPS16
     1  MPSKGPLQSV QVFGRKKTAT AVAHCKRGNG LIKVNGRPLE MIEPRTLQYK LLEPVLLLGK
    61  ERFAGVDIRV RVKGGGHVAQ IYAIRQSISK ALVAYYQKYV DEASKKEIKD ILIQYDRTLL
   121  VADPRRCESK KFGGPGARAR YQKSYR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPS16 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.35
Highest tissue expression
3,512 nTPM

Expression across tissuesHPA

Tissue

  • ovary: 3,512 nTPM
  • pancreas: 2,461 nTPM
  • skeletal muscle: 2,289 nTPM
  • skin: 2,070 nTPM
  • cervix: 1,855 nTPM
  • colon: 1,767 nTPM

Single-cell type

  • esophageal suprabasal cells: 7,901 nCPM
  • esophageal basal cells: 7,379 nCPM
  • extravillous trophoblasts: 7,252 nCPM
  • migrating cytotrophoblasts: 6,609 nCPM
  • esophageal apical cells: 6,126 nCPM
  • gastric progenitor cells: 5,674 nCPM

Immune cell

  • total PBMC: 4,283 nTPM
  • naive CD4 T-cell: 2,540 nTPM
  • memory B-cell: 2,344 nTPM
  • naive B-cell: 2,157 nTPM
  • naive CD8 T-cell: 2,102 nTPM
  • memory CD4 T-cell: 2,030 nTPM

Brain region

  • spinal cord: 415 nTPM
  • white matter: 404 nTPM
  • thalamus: 402 nTPM
  • basal ganglia: 395 nTPM
  • medulla oblongata: 394 nTPM
  • choroid plexus: 379 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.59
gnomAD pLI
0.69
gnomAD missense Z
1.62
DepMap mean gene effect
-2.07
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPS16 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPS16 as an antibody target. Whether an autoantibody or antibody against RPS16 could matter depends on whether native RPS16 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPS16 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPS16 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RPS16. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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