Seroatlas · Human Serome Atlas

RPL3

Large ribosomal subunit protein uL3

Also known as: L3, RL3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P39023
Gene
RPL3
Ensembl
ENSG00000100316
Chromosome
22
Canonical length
403 aa
Protein class
FDA approved drug targets, Plasma proteins, Predicted intracellular proteins, Ribosomal proteins
Subcellular location
Nucleoli,Cytosol

OverviewNCBI Gene

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene's introns. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

403 residues, UniProt reviewed canonical sequence.

>P39023|RPL3
     1  MSHRKFSAPR HGSLGFLPRK RSSRHRGKVK SFPKDDPSKP VHLTAFLGYK AGMTHIVREV
    61  DRPGSKVNKK EVVEAVTIVE TPPMVVVGIV GYVETPRGLR TFKTVFAEHI SDECKRRFYK
   121  NWHKSKKKAF TKYCKKWQDE DGKKQLEKDF SSMKKYCQVI RVIAHTQMRL LPLRQKKAHL
   181  MEIQVNGGTV AEKLDWARER LEQQVPVNQV FGQDEMIDVI GVTKGKGYKG VTSRWHTKKL
   241  PRKTHRGLRK VACIGAWHPA RVAFSVARAG QKGYHHRTEI NKKIYKIGQG YLIKDGKLIK
   301  NNASTDYDLS DKSINPLGGF VHYGEVTNDF VMLKGCVVGT KKRVLTLRKS LLVQTKRRAL
   361  EKIDLKFIDT TSKFGHGRFQ TMEEKKAFMG PLKKDRIAKE EGA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPL3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.3
Highest tissue expression
4,918 nTPM

Expression across tissuesHPA

Tissue

  • ovary: 4,918 nTPM
  • pancreas: 4,506 nTPM
  • skin: 3,373 nTPM
  • bone marrow: 2,796 nTPM
  • cervix: 2,679 nTPM
  • esophagus: 2,560 nTPM

Single-cell type

  • decidual stromal cells: 13,458 nCPM
  • breast secretory cells: 10,258 nCPM
  • esophageal suprabasal cells: 9,730 nCPM
  • esophageal basal cells: 9,240 nCPM
  • epididymal basal cells: 9,162 nCPM
  • extravillous trophoblasts: 8,661 nCPM

Immune cell

  • total PBMC: 11,623 nTPM
  • naive CD4 T-cell: 6,397 nTPM
  • memory B-cell: 5,651 nTPM
  • MAIT T-cell: 5,393 nTPM
  • naive B-cell: 5,138 nTPM
  • naive CD8 T-cell: 5,127 nTPM

Brain region

  • spinal cord: 807 nTPM
  • choroid plexus: 736 nTPM
  • thalamus: 710 nTPM
  • white matter: 680 nTPM
  • medulla oblongata: 657 nTPM
  • basal ganglia: 645 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.24
gnomAD pLI
0.99
gnomAD missense Z
1.56
DepMap mean gene effect
-2.53
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPL3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPL3 as an antibody target. Whether an autoantibody or antibody against RPL3 could matter depends on whether native RPL3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPL3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPL3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RPL3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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