Seroatlas · Human Serome Atlas

RPL4

Large ribosomal subunit protein uL4

Also known as: L4, RL4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P36578
Gene
RPL4
Ensembl
ENSG00000174444
Chromosome
15
Canonical length
427 aa
Protein class
Plasma proteins, Predicted intracellular proteins, Ribosomal proteins
Subcellular location
Nucleoli,Nuclear bodies,Endoplasmic reticulum,Cytosol

OverviewNCBI Gene

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L4E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

427 residues, UniProt reviewed canonical sequence.

>P36578|RPL4
     1  MACARPLISV YSEKGESSGK NVTLPAVFKA PIRPDIVNFV HTNLRKNNRQ PYAVSELAGH
    61  QTSAESWGTG RAVARIPRVR GGGTHRSGQG AFGNMCRGGR MFAPTKTWRR WHRRVNTTQK
   121  RYAICSALAA SALPALVMSK GHRIEEVPEL PLVVEDKVEG YKKTKEAVLL LKKLKAWNDI
   181  KKVYASQRMR AGKGKMRNRR RIQRRGPCII YNEDNGIIKA FRNIPGITLL NVSKLNILKL
   241  APGGHVGRFC IWTESAFRKL DELYGTWRKA ASLKSNYNLP MHKMINTDLS RILKSPEIQR
   301  ALRAPRKKIH RRVLKKNPLK NLRIMLKLNP YAKTMRRNTI LRQARNHKLR VDKAAAAAAA
   361  LQAKSDEKAA VAGKKPVVGK KGKKAAVGVK KQKKPLVGKK AAATKKPAPE KKPAEKKPTT
   421  EEKKPAA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPL4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.41
Highest tissue expression
2,357 nTPM

Expression across tissuesHPA

Tissue

  • ovary: 2,357 nTPM
  • tonsil: 2,291 nTPM
  • breast: 1,895 nTPM
  • bone marrow: 1,816 nTPM
  • tongue: 1,791 nTPM
  • thymus: 1,754 nTPM

Single-cell type

  • extravillous trophoblasts: 4,187 nCPM
  • decidual stromal cells: 3,940 nCPM
  • migrating cytotrophoblasts: 3,127 nCPM
  • esophageal basal cells: 3,105 nCPM
  • esophageal suprabasal cells: 3,042 nCPM
  • esophageal apical cells: 2,337 nCPM

Immune cell

  • total PBMC: 1,759 nTPM
  • naive CD4 T-cell: 891 nTPM
  • naive CD8 T-cell: 724 nTPM
  • memory B-cell: 714 nTPM
  • memory CD4 T-cell: 700 nTPM
  • MAIT T-cell: 668 nTPM

Brain region

  • basal ganglia: 210 nTPM
  • white matter: 210 nTPM
  • hypothalamus: 180 nTPM
  • pons: 174 nTPM
  • medulla oblongata: 173 nTPM
  • hippocampal formation: 171 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.12
gnomAD pLI
1
gnomAD missense Z
1.27
DepMap mean gene effect
-2.22
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPL4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPL4 as an antibody target. Whether an autoantibody or antibody against RPL4 could matter depends on whether native RPL4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPL4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPL4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RPL4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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