Seroatlas · Human Serome Atlas

RPS3A

Small ribosomal subunit protein eS1

Also known as: MFTL, RS3A_HUMAN, S3A

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P61247
Gene
RPS3A
Ensembl
ENSG00000145425
Chromosome
4
Canonical length
264 aa
Protein class
Plasma proteins, Predicted intracellular proteins, Ribosomal proteins
Subcellular location
Nucleoli,Endoplasmic reticulum,Cytosol

OverviewNCBI Gene

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]

Canonical amino-acid sequenceUniProt

264 residues, UniProt reviewed canonical sequence.

>P61247|RPS3A
     1  MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT RTQGTKIASD
    61  GLKGRVFEVS LADLQNDEVA FRKFKLITED VQGKNCLTNF HGMDLTRDKM CSMVKKWQTM
   121  IEAHVDVKTT DGYLLRLFCV GFTKKRNNQI RKTSYAQHQQ VRQIRKKMME IMTREVQTND
   181  LKEVVNKLIP DSIGKDIEKA CQSIYPLHDV FVRKVKMLKK PKFELGKLME LHGEGSSSGK
   241  ATGDETGAKV ERADGYEPPV QESV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPS3A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
5,180 nTPM

Expression across tissuesHPA

Tissue

  • ovary: 5,180 nTPM
  • breast: 4,303 nTPM
  • bone marrow: 4,073 nTPM
  • pancreas: 3,646 nTPM
  • lymph node: 3,125 nTPM
  • cervix: 3,121 nTPM

Single-cell type

  • decidual stromal cells: 8,452 nCPM
  • esophageal suprabasal cells: 8,044 nCPM
  • extravillous trophoblasts: 7,545 nCPM
  • esophageal basal cells: 6,815 nCPM
  • migrating cytotrophoblasts: 5,960 nCPM
  • ovarian stromal cells: 5,867 nCPM

Immune cell

  • total PBMC: 18,558 nTPM
  • naive CD4 T-cell: 8,779 nTPM
  • naive CD8 T-cell: 7,198 nTPM
  • memory B-cell: 7,185 nTPM
  • MAIT T-cell: 6,477 nTPM
  • memory CD4 T-cell: 6,410 nTPM

Brain region

  • spinal cord: 523 nTPM
  • white matter: 486 nTPM
  • thalamus: 425 nTPM
  • basal ganglia: 419 nTPM
  • medulla oblongata: 419 nTPM
  • hypothalamus: 414 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.28
gnomAD pLI
0.97
gnomAD missense Z
2.23
DepMap mean gene effect
-2.73
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Small ribosomal subunit protein eS1
  • Small ribosomal subunit protein eS1, conserved site
  • Small ribosomal subunit protein eS1, eukaryota
  • Ribosomal S3Ae family

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPS3A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPS3A as an antibody target. Whether an autoantibody or antibody against RPS3A could matter depends on whether native RPS3A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPS3A is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPS3A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RPS3A. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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