Seroatlas · Human Serome Atlas

DHX33

ATP-dependent RNA helicase DHX33

Also known as: DDX33, DHX33_HUMAN, DKFZp762F2011, FLJ21972

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9H6R0
Gene
DHX33
Ensembl
ENSG00000005100
Chromosome
17
Canonical length
707 aa
Protein class
Enzymes, Predicted intracellular proteins
Subcellular location
Nucleoli

OverviewNCBI Gene

This gene encodes a member of the DEAD box protein family. The DEAD box proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

Canonical amino-acid sequenceUniProt

707 residues, UniProt reviewed canonical sequence.

>Q9H6R0|DHX33
     1  MPEEAGFPPA KRFRPGSGPP SRAGSFPPGR QVVMLLTAGS GGRGGGGGRR QQPPLAQPSA
    61  SPYPEAVELQ RRSLPIFQAR GQLLAQLRNL DNAVLIGETG SGKTTQIPQY LYEGGISRQG
   121  IIAVTQPRRV AAISLATRVS DEKRTELGKL VGYTVRFDDV TSEDTRIKFL TDGMLLREAI
   181  SDSLLRKYSC VILDEAHERT IHTDVLFGVV KAAQKRRKEL GKLPLKVIVM SATMDVDLFS
   241  QYFNGAPVLY LEGRQHPIQV FYTKQPQNDY LHAALVSVFQ IHQEAPSSQD ILVFLTGQEE
   301  IEAMSKTCRD IAKHLPDGCP AMLVLPLYAS LPYAQQLRVF QGAPKGYRKV IISTNIAETS
   361  ITITGIKYVV DTGMVKAKKY NPDSGLEVLA VQRVSKTQAW QRTGRAGRED SGICYRLYTE
   421  DEFEKFDKMT VPEIQRCNLA SVMLQLLAMK VPNVLTFDFM SKPSPDHIQA AIAQLDLLGA
   481  LEHKDDQLTL TPMGRKMAAF PLEPKFAKTI LMSPKFHCTE EILTIVSLLS VDSVLHNPPS
   541  RREEVQGVRK KFISSEGDHM TLLNIYRTFK NLGGNKDWCK ENFVNSKNMT LVAEVRAQLR
   601  DICLKMSMPI ASSRGDVESV RRCLAHSLFM STAELQPDGT YATTDTHQPV AIHPSSVLFH
   661  CKPACVVYTE LLYTNKCYMR DLCVIDAQWL YEAAPEYFRR KLRTARN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DHX33 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.26
Highest tissue expression
7.7 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 7.7 nTPM
  • liver: 7.6 nTPM
  • cerebral cortex: 7.5 nTPM
  • cerebellum: 7.4 nTPM
  • parathyroid gland: 7.2 nTPM
  • breast: 6.5 nTPM

Single-cell type

  • adrenal medulla cells: 21 nCPM
  • erythrocyte progenitors: 21 nCPM
  • megakaryocyte progenitors: 17 nCPM
  • adrenal cortex cells: 15 nCPM
  • megakaryocyte-erythroid progenitors: 14 nCPM
  • myonuclei: 14 nCPM

Immune cell

  • classical monocyte: 0.9 nTPM
  • plasmacytoid DC: 0.9 nTPM
  • naive B-cell: 0.8 nTPM
  • NK-cell: 0.8 nTPM
  • memory B-cell: 0.7 nTPM
  • memory CD4 T-cell: 0.6 nTPM

Brain region

  • basal ganglia: 11 nTPM
  • cerebral cortex: 11 nTPM
  • thalamus: 11 nTPM
  • hippocampal formation: 11 nTPM
  • white matter: 11 nTPM
  • amygdala: 10 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.68
gnomAD pLI
0
gnomAD missense Z
0.46
DepMap mean gene effect
-1.17
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DHX33 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DHX33 as an antibody target. Whether an autoantibody or antibody against DHX33 could matter depends on whether native DHX33 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DHX33 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DHX33 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DHX33. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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