Seroatlas · Human Serome Atlas

RPL28

Large ribosomal subunit protein eL28

Also known as: FLJ43307, L28, RL28_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P46779
Gene
RPL28
Ensembl
ENSG00000108107
Chromosome
19
Canonical length
137 aa
Protein class
Predicted intracellular proteins, Ribosomal proteins
Subcellular location
Endoplasmic reticulum,Cytosol

OverviewNCBI Gene

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L28E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2008]

Canonical amino-acid sequenceUniProt

137 residues, UniProt reviewed canonical sequence.

>P46779|RPL28
     1  MSAHLQWMVV RNCSSFLIKR NKQTYSTEPN NLKARNSFRY NGLIHRKTVG VEPAADGKGV
    61  VVVIKRRSGQ RKPATSYVRT TINKNARATL SSIRHMIRKN KYRPDLRMAA IRRASAILRS
   121  QKPVMVKRKR TRPTKSS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPL28 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.38
Highest tissue expression
2,639 nTPM

Expression across tissuesHPA

Tissue

  • ovary: 2,639 nTPM
  • esophagus: 2,613 nTPM
  • skin: 2,517 nTPM
  • pancreas: 1,917 nTPM
  • spleen: 1,901 nTPM
  • vagina: 1,845 nTPM

Single-cell type

  • esophageal apical cells: 15,121 nCPM
  • esophageal suprabasal cells: 11,054 nCPM
  • esophageal basal cells: 8,304 nCPM
  • decidual stromal cells: 7,510 nCPM
  • extravillous trophoblasts: 7,172 nCPM
  • enteric transient amplifying cells: 6,647 nCPM

Immune cell

  • total PBMC: 3,401 nTPM
  • memory B-cell: 1,960 nTPM
  • naive B-cell: 1,938 nTPM
  • naive CD4 T-cell: 1,900 nTPM
  • naive CD8 T-cell: 1,741 nTPM
  • T-reg: 1,732 nTPM

Brain region

  • medulla oblongata: 624 nTPM
  • white matter: 557 nTPM
  • pons: 546 nTPM
  • hypothalamus: 525 nTPM
  • thalamus: 516 nTPM
  • spinal cord: 514 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.62
gnomAD pLI
0.77
gnomAD missense Z
0.07
DepMap mean gene effect
-0.8
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPL28 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPL28 as an antibody target. Whether an autoantibody or antibody against RPL28 could matter depends on whether native RPL28 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPL28 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPL28 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RPL28. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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