Seroatlas · Human Serome Atlas

POLR2E

DNA-directed RNA polymerases I, II, and III subunit RPABC1

Also known as: hRPB25, hsRPB5, RPAB1_HUMAN, RPABC1, RPB5, XAP4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P19388
Gene: POLR2E
Ensembl: ENSG00000099817
Chromosome: 19
Canonical length: 210 aa
Protein class: Metabolic proteins, Predicted intracellular proteins, RNA polymerase related proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Canonical amino-acid sequenceUniProt

210 residues, UniProt reviewed canonical sequence.

>P19388|POLR2E
     1  MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQSGDKPSE GRPRRTDLTV
    61  LVAHNDDPTD QMFVFFPEEP KVGIKTIKVY CQRMQEENIT RALIVVQQGM TPSAKQSLVD
   121  MAPKYILEQF LQQELLINIT EHELVPEHVV MTKEEVTELL ARYKLRENQL PRIQAGDPVA
   181  RYFGIKRGQV VKIIRPSETA GRYITYRLVQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POLR2E can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.3
Highest tissue expression: 41 nTPM

Expression across tissuesHPA

Tissue

liver: 41 nTPM
cerebral cortex: 30 nTPM
adipose tissue: 29 nTPM
hippocampal formation: 28 nTPM
amygdala: 27 nTPM
basal ganglia: 25 nTPM

Single-cell type

oocytes: 479 nCPM
hofbauer cells: 345 nCPM
esophageal suprabasal cells: 338 nCPM
esophageal basal cells: 335 nCPM
migrating cytotrophoblasts: 321 nCPM
extravillous trophoblasts: 297 nCPM

Immune cell

classical monocyte: 51 nTPM
myeloid DC: 45 nTPM
total PBMC: 39 nTPM
non-classical monocyte: 39 nTPM
intermediate monocyte: 34 nTPM
plasmacytoid DC: 31 nTPM

Brain region

cerebral cortex: 45 nTPM
pons: 45 nTPM
medulla oblongata: 41 nTPM
thalamus: 40 nTPM
hippocampal formation: 39 nTPM
amygdala: 38 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about POLR2E.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 49 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Short stature

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.49
gnomAD pLI: 0
gnomAD missense Z: 0.35
DepMap mean gene effect: -1.45
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

RNA polymerase, subunit H/Rpb5 C-terminal
RNA polymerase, Rpb5, N-terminal
DNA-directed RNA polymerase subunit Rpo5/Rpb5
RNA polymerase, subunit H/Rpb5, conserved site
RPB5-like RNA polymerase subunit superfamily
RNA polymerase Rpb5, N-terminal domain superfamily
RNA polymerase Rpb5, C-terminal domain
RNA polymerase Rpb5, N-terminal domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of POLR2E in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POLR2E as an antibody target. Whether an autoantibody or antibody against POLR2E could matter depends on whether native POLR2E is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POLR2E is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POLR2E as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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