Seroatlas · Human Serome Atlas

MED31

Mediator of RNA polymerase II transcription subunit 31

Also known as: CGI-125, MED31_HUMAN, Soh1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y3C7
Gene: MED31
Ensembl: ENSG00000108590
Chromosome: 17
Canonical length: 131 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Predicted to enable transcription coregulator activity and ubiquitin protein ligase activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to act upstream of or within limb development and negative regulation of fibroblast proliferation. Located in nucleus. Part of core mediator complex. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

131 residues, UniProt reviewed canonical sequence.

>Q9Y3C7|MED31
     1  MAAAVAMETD DAGNRLRFQL ELEFVQCLAN PNYLNFLAQR GYFKDKAFVN YLKYLLYWKD
    61  PEYAKYLKYP QCLHMLELLQ YEHFRKELVN AQCAKFIDEQ QILHWQHYSR KRMRLQQALA
   121  EQQQQNNTSG K

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MED31 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.37
Highest tissue expression: 25 nTPM

Expression across tissuesHPA

Tissue

epididymis: 25 nTPM
pituitary gland: 24 nTPM
esophagus: 24 nTPM
basal ganglia: 23 nTPM
hypothalamus: 23 nTPM
cerebral cortex: 22 nTPM

Single-cell type

esophageal apical cells: 241 nCPM
esophageal suprabasal cells: 126 nCPM
early primary spermatocytes: 124 nCPM
suprabasal keratinocytes: 119 nCPM
gastric progenitor cells: 108 nCPM
late spermatids: 102 nCPM

Immune cell

basophil: 78 nTPM
neutrophil: 64 nTPM
eosinophil: 39 nTPM
T-reg: 31 nTPM
gdT-cell: 26 nTPM
memory CD8 T-cell: 26 nTPM

Brain region

hypothalamus: 19 nTPM
basal ganglia: 18 nTPM
pons: 17 nTPM
hippocampal formation: 15 nTPM
midbrain: 15 nTPM
cerebral cortex: 14 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.28
gnomAD pLI: 0
gnomAD missense Z: 1.32
DepMap mean gene effect: -1.47
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Mediator complex, subunit Med31
Mediator of RNA polymerase II, subunit Med31 superfamily
SOH1

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MED31 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MED31 as an antibody target. Whether an autoantibody or antibody against MED31 could matter depends on whether native MED31 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MED31 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MED31 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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