Seroatlas · Human Serome Atlas

MED6

Mediator of RNA polymerase II transcription subunit 6

Also known as: MED6_HUMAN, NY-REN-28

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O75586
Gene: MED6
Ensembl: ENSG00000133997
Chromosome: 14
Canonical length: 246 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Enables transcription coactivator activity and transcription coactivator binding activity. Acts upstream of or within positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of core mediator complex and mediator complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

246 residues, UniProt reviewed canonical sequence.

>O75586|MED6
     1  MAAVDIRDNL LGISWVDSSW IPILNSGSVL DYFSERSNPF YDRTCNNEVV KMQRLTLEHL
    61  NQMVGIEYIL LHAQEPILFI IRKQQRQSPA QVIPLADYYI IAGVIYQAPD LGSVINSRVL
   121  TAVHGIQSAF DEAMSYCRYH PSKGYWWHFK DHEEQDKVRP KAKRKEEPSS IFQRQRVDAL
   181  LLDLRQKFPP KFVQLKPGEK PVPVDQTKKE AEPIPETVKP EEKETTKNVQ QTVSAKGPPE
   241  KRMRLQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MED6 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.48
Highest tissue expression: 25 nTPM

Expression across tissuesHPA

Tissue

tonsil: 25 nTPM
lymph node: 23 nTPM
thymus: 23 nTPM
skeletal muscle: 23 nTPM
tongue: 21 nTPM
retina: 20 nTPM

Single-cell type

esophageal apical cells: 122 nCPM
syncytiotrophoblasts: 63 nCPM
rod photoreceptor cells: 61 nCPM
extravillous trophoblasts: 61 nCPM
oocytes: 60 nCPM
migrating cytotrophoblasts: 53 nCPM

Immune cell

eosinophil: 25 nTPM
T-reg: 23 nTPM
memory CD4 T-cell: 23 nTPM
non-classical monocyte: 23 nTPM
NK-cell: 23 nTPM
naive CD8 T-cell: 22 nTPM

Brain region

cerebellum: 57 nTPM
cerebral cortex: 41 nTPM
hypothalamus: 39 nTPM
basal ganglia: 37 nTPM
white matter: 37 nTPM
midbrain: 35 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1
gnomAD pLI: 0
gnomAD missense Z: 0.96
DepMap mean gene effect: -1.75
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Mediator complex, subunit Med6
Mediator complex, subunit Med6, metazoa/plant
Mediator complex, subunit Med6 superfamily
MED6 mediator sub complex component

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MED6 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MED6 as an antibody target. Whether an autoantibody or antibody against MED6 could matter depends on whether native MED6 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MED6 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MED6 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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