Seroatlas · Human Serome Atlas

MED28

Mediator of RNA polymerase II transcription subunit 28

Also known as: DKFZP434N185, EG1, magicin, MED28_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H204
Gene: MED28
Ensembl: ENSG00000118579
Chromosome: 4
Canonical length: 178 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Predicted to enable actin binding activity. Predicted to be involved in RNA polymerase II preinitiation complex assembly; positive regulation of transcription elongation by RNA polymerase II; and positive regulation of transcription initiation by RNA polymerase II. Predicted to act upstream of or within negative regulation of smooth muscle cell differentiation and somatic stem cell population maintenance. Located in nucleoplasm. Part of core mediator complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

178 residues, UniProt reviewed canonical sequence.

>Q9H204|MED28
     1  MAAPLGGMFS GQPPGPPQAP PGLPGQASLL QAAPGAPRPS SSTLVDELES SFEACFASLV
    61  SQDYVNGTDQ EEIRTGVDQC IQKFLDIARQ TECFFLQKRL QLSVQKPEQV IKEDVSELRN
   121  ELQRKDALVQ KHLTKLRHWQ QVLEDINVQH KKPADIPQGS LAYLEQASAN IPAPLKPT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MED28 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.54
Highest tissue expression: 14 nTPM

Expression across tissuesHPA

Tissue

ovary: 14 nTPM
skeletal muscle: 12 nTPM
cervix: 12 nTPM
bone marrow: 12 nTPM
endometrium: 11 nTPM
cerebellum: 11 nTPM

Single-cell type

extravillous trophoblasts: 203 nCPM
migrating cytotrophoblasts: 172 nCPM
syncytiotrophoblasts: 146 nCPM
esophageal apical cells: 139 nCPM
cytotrophoblasts: 131 nCPM
hofbauer cells: 123 nCPM

Immune cell

neutrophil: 8.9 nTPM
total PBMC: 7.4 nTPM
classical monocyte: 6.6 nTPM
eosinophil: 6.5 nTPM
myeloid DC: 6.4 nTPM
naive CD4 T-cell: 6.4 nTPM

Brain region

white matter: 30 nTPM
medulla oblongata: 26 nTPM
hypothalamus: 26 nTPM
thalamus: 26 nTPM
cerebellum: 25 nTPM
basal ganglia: 25 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.4
gnomAD pLI: 0
gnomAD missense Z: -0.28
DepMap mean gene effect: -1.24
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

actin binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

Mediator complex, subunit Med28
Mediator complex subunit 28

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MED28 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MED28 as an antibody target. Whether an autoantibody or antibody against MED28 could matter depends on whether native MED28 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MED28 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MED28 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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