MED29
Mediator of RNA polymerase II transcription subunit 29
Also known as: DKFZp434H247, IXL, MED2, MED29_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q9NX70
- Gene
- MED29
- Ensembl
- ENSG00000063322
- Chromosome
- 19
- Canonical length
- 200 aa
- Protein class
- Predicted intracellular proteins
- Subcellular location
- Nucleoplasm
OverviewNCBI Gene
MED29 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors (Sato et al., 2003 [PubMed 14576168]).[supplied by OMIM, Aug 2009]
Canonical amino-acid sequenceUniProt
200 residues, UniProt reviewed canonical sequence.
>Q9NX70|MED29
1 MAASQQQASA ASSAAGVSGP SSAGGPGPQQ QPQPPAQLVG PAQSGLLQQQ QQDFDPVQRY
61 KMLIPQLKES LQTLMKVAAQ NLIQNTNIDN GQKSSDGPIQ RFDKCLEEFY ALCDQLELCL
121 RLAHECLSQS CDSAKHSPTL VPTATKPDAV QPDSLPYPQY LAVIKAQISC AKDIHTALLD
181 CANKVTGKTP APPAGPGGTLLocalizationUniProt · AlphaFold · HPA
Whether an antibody against MED29 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.55
- Highest tissue expression
- 56 nTPM
Expression across tissuesHPA
Tissue
- cerebral cortex: 56 nTPM
- amygdala: 53 nTPM
- basal ganglia: 52 nTPM
- midbrain: 48 nTPM
- hypothalamus: 45 nTPM
- hippocampal formation: 45 nTPM
Single-cell type
- enterocytes: 150 nCPM
- breast myoepithelial cells: 148 nCPM
- breast lactating cells: 106 nCPM
- colonocytes: 103 nCPM
- neutrophils: 99 nCPM
- late spermatids: 98 nCPM
Immune cell
- non-classical monocyte: 78 nTPM
- intermediate monocyte: 60 nTPM
- neutrophil: 55 nTPM
- classical monocyte: 49 nTPM
- basophil: 48 nTPM
- total PBMC: 47 nTPM
Brain region
- thalamus: 61 nTPM
- medulla oblongata: 56 nTPM
- midbrain: 55 nTPM
- basal ganglia: 54 nTPM
- amygdala: 52 nTPM
- white matter: 52 nTPM
DiseaseUniProt · ClinVar · IEDB · PubMed
Four sources answering four different questions about MED29.
Disease | GeneticClinVar
1 pathogenic / likely-pathogenic of 39 ClinVar records.
Conditions with pathogenic or likely-pathogenic variants.
- pontocerebellar hypoplasia with cataract
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.18
- gnomAD pLI
- 0
- gnomAD missense Z
- -0.33
- DepMap mean gene effect
- -0.87
- DepMap dependency class
- common
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- positive regulation of transcription elongation by RNA polymerase II
- positive regulation of transcription initiation by RNA polymerase II
- regulation of transcription by RNA polymerase II
- RNA polymerase II preinitiation complex assembly
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Mediator complex, subunit Med29, metazoa
- Mediator complex subunit 29
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of MED29 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads MED29 as an antibody target. Whether an autoantibody or antibody against MED29 could matter depends on whether native MED29 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
MED29 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label MED29 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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