GTF2F1
General transcription factor IIF subunit 1
Also known as: BTF4, RAP74, T2FA_HUMAN, TF2F1, TFIIF
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P35269
- Gene
- GTF2F1
- Ensembl
- ENSG00000125651
- Chromosome
- 19
- Canonical length
- 517 aa
- Protein class
- Plasma proteins, Predicted intracellular proteins
- Subcellular location
- Nucleoplasm
OverviewNCBI Gene
Enables several functions, including RNA polymerase II general transcription initiation factor activity; phosphatase activator activity; and promoter-specific chromatin binding activity. Involved in positive regulation of transcription by RNA polymerase II; response to virus; and transcription by RNA polymerase II. Located in cell junction and nucleoplasm. Part of transcription factor TFIID complex and transcription factor TFIIF complex. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
517 residues, UniProt reviewed canonical sequence.
>P35269|GTF2F1
1 MAALGPSSQN VTEYVVRVPK NTTKKYNIMA FNAADKVNFA TWNQARLERD LSNKKIYQEE
61 EMPESGAGSE FNRKLREEAR RKKYGIVLKE FRPEDQPWLL RVNGKSGRKF KGIKKGGVTE
121 NTSYYIFTQC PDGAFEAFPV HNWYNFTPLA RHRTLTAEEA EEEWERRNKV LNHFSIMQQR
181 RLKDQDQDED EEEKEKRGRR KASELRIHDL EDDLEMSSDA SDASGEEGGR VPKAKKKAPL
241 AKGGRKKKKK KGSDDEAFED SDDGDFEGQE VDYMSDGSSS SQEEPESKAK APQQEEGPKG
301 VDEQSDSSEE SEEEKPPEED KEEEEEKKAP TPQEKKRRKD SSEESDSSEE SDIDSEASSA
361 LFMAKKKTPP KRERKPSGGS SRGNSRPGTP SAEGGSTSST LRAAASKLEQ GKRVSEMPAA
421 KRLRLDTGPQ SLSGKSTPQP PSGKTTPNSG DVQVTEDAVR RYLTRKPMTT KDLLKKFQTK
481 KTGLSSEQTV NVLAQILKRL NPERKMINDK MHFSLKELocalizationUniProt · AlphaFold · HPA
Whether an antibody against GTF2F1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.57
- Highest tissue expression
- 86 nTPM
Expression across tissuesHPA
Tissue
- cerebellum: 86 nTPM
- testis: 68 nTPM
- bone marrow: 67 nTPM
- ovary: 64 nTPM
- pancreas: 58 nTPM
- cerebral cortex: 54 nTPM
Single-cell type
- late spermatids: 673 nCPM
- early spermatids: 272 nCPM
- late primary spermatocytes: 145 nCPM
- esophageal apical cells: 145 nCPM
- erythrocyte progenitors: 89 nCPM
- megakaryocytes: 87 nCPM
Immune cell
- neutrophil: 40 nTPM
- memory B-cell: 33 nTPM
- naive B-cell: 32 nTPM
- non-classical monocyte: 31 nTPM
- plasmacytoid DC: 30 nTPM
- MAIT T-cell: 30 nTPM
Brain region
- cerebellum: 72 nTPM
- cerebral cortex: 70 nTPM
- hippocampal formation: 60 nTPM
- thalamus: 59 nTPM
- basal ganglia: 58 nTPM
- amygdala: 58 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.76
- gnomAD pLI
- 0
- gnomAD missense Z
- 0.41
- DepMap mean gene effect
- -0.59
- DepMap dependency class
- common
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- negative regulation of protein binding
- positive regulation of transcription by RNA polymerase II
- positive regulation of transcription elongation by RNA polymerase II
- response to virus
- transcription by RNA polymerase II
- transcription elongation by RNA polymerase II
- transcription initiation at RNA polymerase II promoter
Molecular functions
- DNA binding
- phosphatase activator activity
- promoter-specific chromatin binding
- protein domain specific binding
- protein phosphatase binding
- RNA binding
- RNA polymerase II general transcription initiation factor activity
- RNA polymerase II general transcription initiation factor binding
- TFIIF-class transcription factor complex binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Transcription Factor IIF, Rap30/Rap74, interaction
- Winged helix-like DNA-binding domain superfamily
- Winged helix DNA-binding domain superfamily
- Transcription initiation factor IIF, alpha subunit
- Transcription initiation factor IIF, alpha subunit (TFIIF-alpha)
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of GTF2F1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads GTF2F1 as an antibody target. Whether an autoantibody or antibody against GTF2F1 could matter depends on whether native GTF2F1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
GTF2F1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label GTF2F1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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