Seroatlas · Human Serome Atlas

MED27

Mediator of RNA polymerase II transcription subunit 27

Also known as: CRSP34, CRSP8, MED27_HUMAN, MED3, TRAP37

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q6P2C8
Gene
MED27
Ensembl
ENSG00000160563
Chromosome
9
Canonical length
311 aa
Protein class
Disease related genes, Human disease related genes, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nucleoli,Cytosol

OverviewNCBI Gene

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

Canonical amino-acid sequenceUniProt

311 residues, UniProt reviewed canonical sequence.

>Q6P2C8|MED27
     1  MADVINVSVN LEAFSQAISA IQALRSSVSR VFDCLKDGMR NKETLEGREK AFIAHFQDNL
    61  HSVNRDLNEL ERLSNLVGKP SENHPLHNSG LLSLDPVQDK TPLYSQLLQA YKWSNKLQYH
   121  AGLASGLLNQ QSLKRSANQM GVSAKRRPKA QPTTLVLPPQ YVDDVISRID RMFPEMSIHL
   181  SRPNGTSAML LVTLGKVLKV IVVMRSLFID RTIVKGYNEN VYTEDGKLDI WSKSNYQVFQ
   241  KVTDHATTAL LHYQLPQMPD VVVRSFMTWL RSYIKLFQAP CQRCGKFLQD GLPPTWRDFR
   301  TLEAFHDTCR Q

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MED27 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.38
Highest tissue expression
29 nTPM

Expression across tissuesHPA

Tissue

  • blood vessel: 29 nTPM
  • thymus: 26 nTPM
  • epididymis: 22 nTPM
  • skeletal muscle: 21 nTPM
  • skin: 20 nTPM
  • hippocampal formation: 19 nTPM

Single-cell type

  • podocytes: 318 nCPM
  • pituicytes/fscs: 187 nCPM
  • cone photoreceptor cells: 184 nCPM
  • choroid plexus epithelial cells: 173 nCPM
  • oligodendrocytes: 170 nCPM
  • syncytiotrophoblasts: 157 nCPM

Immune cell

  • T-reg: 30 nTPM
  • plasmacytoid DC: 29 nTPM
  • intermediate monocyte: 29 nTPM
  • myeloid DC: 28 nTPM
  • NK-cell: 28 nTPM
  • gdT-cell: 27 nTPM

Brain region

  • hippocampal formation: 29 nTPM
  • cerebral cortex: 29 nTPM
  • white matter: 26 nTPM
  • medulla oblongata: 24 nTPM
  • amygdala: 24 nTPM
  • basal ganglia: 23 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MED27.

Disease | AllUniProt

Conditions MED27 is implicated in, by any mechanism.

  • Neurodevelopmental disorder with spasticity, cataracts, and cerebellar hypoplasia (NEDSCAC) MIM:619286

Disease | GeneticClinVar

3 pathogenic / likely-pathogenic of 62 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Neurodevelopmental disorder with spasticity, cataracts, and cerebellar hypoplasia

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.02
gnomAD pLI
0
gnomAD missense Z
1.26
DepMap mean gene effect
-1.05
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Mediator complex, subunit Med27
  • Mediator complex subunit 27

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MED27 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MED27 as an antibody target. Whether an autoantibody or antibody against MED27 could matter depends on whether native MED27 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MED27 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MED27 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MED27. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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