GTF2B
Transcription initiation factor IIB
Also known as: TF2B_HUMAN, TFIIB
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q00403
- Gene
- GTF2B
- Ensembl
- ENSG00000137947
- Chromosome
- 1
- Canonical length
- 316 aa
- Protein class
- Enzymes, Plasma proteins, Predicted intracellular proteins
- Subcellular location
- Nucleoplasm,Nuclear bodies
OverviewNCBI Gene
This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
316 residues, UniProt reviewed canonical sequence.
>Q00403|GTF2B
1 MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS EWRTFSNDKA
61 TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK YQNRRTMSSS DRAMMNAFKE
121 ITTMADRINL PRNIVDRTNN LFKQVYEQKS LKGRANDAIA SACLYIACRQ EGVPRTFKEI
181 CAVSRISKKE IGRCFKLILK ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV
241 ELDLVPGRSP ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF
301 PTDFKFDTPV DKLPQLLocalizationUniProt · AlphaFold · HPA
Whether an antibody against GTF2B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.35
- Highest tissue expression
- 59 nTPM
Expression across tissuesHPA
Tissue
- bone marrow: 59 nTPM
- thymus: 38 nTPM
- choroid plexus: 36 nTPM
- liver: 31 nTPM
- tonsil: 29 nTPM
- urinary bladder: 29 nTPM
Single-cell type
- syncytiotrophoblasts: 229 nCPM
- neutrophils: 222 nCPM
- pdcs: 193 nCPM
- epididymal basal cells: 184 nCPM
- t-cells: 176 nCPM
- oocytes: 175 nCPM
Immune cell
- neutrophil: 100 nTPM
- basophil: 92 nTPM
- eosinophil: 82 nTPM
- T-reg: 70 nTPM
- naive CD4 T-cell: 63 nTPM
- myeloid DC: 63 nTPM
Brain region
- choroid plexus: 18 nTPM
- white matter: 16 nTPM
- hypothalamus: 16 nTPM
- cerebellum: 15 nTPM
- spinal cord: 15 nTPM
- midbrain: 14 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.92
- gnomAD pLI
- 0
- gnomAD missense Z
- 2.53
- DepMap mean gene effect
- -1.89
- DepMap dependency class
- pan
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- DNA-templated transcription initiation
- meiotic sister chromatid cohesion
- positive regulation of core promoter binding
- protein acetylation
- RNA polymerase II core complex assembly
- RNA polymerase II preinitiation complex assembly
- spindle assembly
- transcription by RNA polymerase II
- transcription initiation at RNA polymerase II promoter
- transcriptional start site selection at RNA polymerase II promoter
- viral transcription
Molecular functions
- acetyltransferase activity
- DNA-binding transcription factor binding
- nuclear thyroid hormone receptor binding
- promoter-specific chromatin binding
- protein-lysine-acetyltransferase activity
- RNA polymerase II complex binding
- RNA polymerase II core promoter sequence-specific DNA binding
- RNA polymerase II general transcription initiation factor activity
- TBP-class protein binding
- zinc ion binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of GTF2B in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads GTF2B as an antibody target. Whether an autoantibody or antibody against GTF2B could matter depends on whether native GTF2B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
GTF2B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label GTF2B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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