Seroatlas · Human Serome Atlas

MED30

Mediator of RNA polymerase II transcription subunit 30

Also known as: MED30_HUMAN, THRAP6, TRAP25

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96HR3
Gene: MED30
Ensembl: ENSG00000164758
Chromosome: 8
Canonical length: 178 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).[supplied by OMIM, Nov 2010]

Canonical amino-acid sequenceUniProt

178 residues, UniProt reviewed canonical sequence.

>Q96HR3|MED30
     1  MSTPPLAASG MAPGPFAGPQ AQQAAREVNT ASLCRIGQET VQDIVYRTME IFQLLRNMQL
    61  PNGVTYHTGT YQDRLTKLQD NLRQLSVLFR KLRLVYDKCN ENCGGMDPIP VEQLIPYVEE
   121  DGSKNDDRAG PPRFASEERR EIAEVNKKLK QKNQQLKQIM DQLRNLIWDI NAMLAMRN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MED30 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.52
Highest tissue expression: 42 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 42 nTPM
thymus: 23 nTPM
blood vessel: 19 nTPM
ovary: 19 nTPM
adipose tissue: 18 nTPM
breast: 17 nTPM

Single-cell type

basal keratinocytes: 131 nCPM
gastric progenitor cells: 108 nCPM
suprabasal keratinocytes: 101 nCPM
neutrophil progenitors: 92 nCPM
extravillous trophoblasts: 91 nCPM
fallopian tube ciliated cells: 83 nCPM

Immune cell

eosinophil: 112 nTPM
neutrophil: 91 nTPM
plasmacytoid DC: 83 nTPM
non-classical monocyte: 70 nTPM
basophil: 65 nTPM
naive B-cell: 64 nTPM

Brain region

white matter: 9.1 nTPM
spinal cord: 6.9 nTPM
cerebellum: 6.8 nTPM
basal ganglia: 6.6 nTPM
medulla oblongata: 6.1 nTPM
pons: 5.7 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.67
gnomAD pLI: 0.4
gnomAD missense Z: 0.69
DepMap mean gene effect: -1.92
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 15% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Mediator complex, subunit Med30, metazoa
Mediator complex subunit 30

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MED30 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MED30 as an antibody target. Whether an autoantibody or antibody against MED30 could matter depends on whether native MED30 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MED30 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MED30 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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