Seroatlas · Human Serome Atlas

MED21

Mediator of RNA polymerase II transcription subunit 21

Also known as: MED21_HUMAN, SRB7, SURB7

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q13503
Gene: MED21
Ensembl: ENSG00000152944
Chromosome: 12
Canonical length: 144 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli,Cytosol

OverviewNCBI Gene

This gene encodes a member of the mediator complex subunit 21 family. The encoded protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

Canonical amino-acid sequenceUniProt

144 residues, UniProt reviewed canonical sequence.

>Q13503|MED21
     1  MADRLTQLQD AVNSLADQFC NAIGVLQQCG PPASFNNIQT AINKDQPANP TEEYAQLFAA
    61  LIARTAKDID VLIDSLPSEE STAALQAASL YKLEEENHEA ATCLEDVVYR GDMLLEKIQS
   121  ALADIAQSQL KTRSGTHSQS LPDS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MED21 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.48
Highest tissue expression: 33 nTPM

Expression across tissuesHPA

Tissue

tongue: 33 nTPM
testis: 28 nTPM
bone marrow: 27 nTPM
kidney: 24 nTPM
parathyroid gland: 24 nTPM
heart muscle: 24 nTPM

Single-cell type

late primary spermatocytes: 308 nCPM
early spermatids: 240 nCPM
esophageal apical cells: 187 nCPM
suprabasal keratinocytes: 157 nCPM
esophageal suprabasal cells: 153 nCPM
oocytes: 152 nCPM

Immune cell

basophil: 56 nTPM
naive CD4 T-cell: 35 nTPM
T-reg: 35 nTPM
eosinophil: 34 nTPM
memory CD8 T-cell: 31 nTPM
naive CD8 T-cell: 31 nTPM

Brain region

cerebellum: 30 nTPM
cerebral cortex: 27 nTPM
hypothalamus: 24 nTPM
choroid plexus: 24 nTPM
basal ganglia: 24 nTPM
hippocampal formation: 23 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.12
gnomAD pLI: 0.01
gnomAD missense Z: 0.58
DepMap mean gene effect: -1.01
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Mediator complex, subunit Med7/Med21-like
Mediator complex, subunit Med21
Subunit 21 of Mediator complex

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MED21 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MED21 as an antibody target. Whether an autoantibody or antibody against MED21 could matter depends on whether native MED21 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MED21 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MED21 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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