Seroatlas · Human Serome Atlas

MED14

Mediator of RNA polymerase II transcription subunit 14

Also known as: CRSP150, CRSP2, CSRP, CXorf4, EXLM1, MED14_HUMAN, RGR1, TRAP170

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O60244
Gene
MED14
Ensembl
ENSG00000180182
Chromosome
X
Canonical length
1454 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nuclear bodies,Vesicles

OverviewNCBI Gene

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1454 residues, UniProt reviewed canonical sequence.

>O60244|MED14
     1  MAPVQLENHQ LVPPGGGGGG SGGPPSAPAP PPPGAAVAAA AAAAASPGYR LSTLIEFLLH
    61  RAYSELMVLT DLLPRKSDVE RKIEIVQFAS RTRQLFVRLL ALVKWANNAG KVEKCAMISS
   121  FLDQQAILFV DTADRLASLA RDALVHARLP SFAIPYAIDV LTTGSYPRLP TCIRDKIIPP
   181  DPITKIEKQA TLHQLNQILR HRLVTTDLPP QLANLTVANG RVKFRVEGEF EATLTVMGDD
   241  PDVPWRLLKL EILVEDKETG DGRALVHSMQ ISFIHQLVQS RLFADEKPLQ DMYNCLHSFC
   301  LSLQLEVLHS QTLMLIRERW GDLVQVERYH AGKCLSLSVW NQQVLGRKTG TASVHKVTIK
   361  IDENDVSKPL QIFHDPPLPA SDSKLVERAM KIDHLSIEKL LIDSVHARAH QKLQELKAIL
   421  RGFNANENSS IETALPALVV PILEPCGNSE CLHIFVDLHS GMFQLMLYGL DQATLDDMEK
   481  SVNDDMKRII PWIQQLKFWL GQQRCKQSIK HLPTISSETL QLSNYSTHPI GNLSKNKLFI
   541  KLTRLPQYYI VVEMLEVPNK PTQLSYKYYF MSVNAADRED SPAMALLLQQ FKENIQDLVF
   601  RTKTGKQTRT NAKRKLSDDP CPVESKKTKR AGEMCAFNKV LAHFVAMCDT NMPFVGLRLE
   661  LSNLEIPHQG VQVEGDGFSH AIRLLKIPPC KGITEETQKA LDRSLLDCTF RLQGRNNRTW
   721  VAELVFANCP LNGTSTREQG PSRHVYLTYE NLLSEPVGGR KVVEMFLNDW NSIARLYECV
   781  LEFARSLPDI PAHLNIFSEV RVYNYRKLIL CYGTTKGSSI SIQWNSIHQK FHISLGTVGP
   841  NSGCSNCHNT ILHQLQEMFN KTPNVVQLLQ VLFDTQAPLN AINKLPTVPM LGLTQRTNTA
   901  YQCFSILPQS STHIRLAFRN MYCIDIYCRS RGVVAIRDGA YSLFDNSKLV EGFYPAPGLK
   961  TFLNMFVDSN QDARRRSVNE DDNPPSPIGG DMMDSLISQL QPPPQQQPFP KQPGTSGAYP
  1021  LTSPPTSYHS TVNQSPSMMH TQSPGNLHAA SSPSGALRAP SPASFVPTPP PSSHGISIGP
  1081  GASFASPHGT LDPSSPYTMV SPSGRAGNWP GSPQVSGPSP AARMPGMSPA NPSLHSPVPD
  1141  ASHSPRAGTS SQTMPTNMPP PRKLPQRSWA ASIPTILTHS ALNILLLPSP TPGLVPGLAG
  1201  SYLCSPLERF LGSVIMRRHL QRIIQQETLQ LINSNEPGVI MFKTDALKCR VALSPKTNQT
  1261  LQLKVTPENA GQWKPDELQV LEKFFETRVA GPPFKANTLI AFTKLLGAPT HILRDCVHIM
  1321  KLELFPDQAT QLKWNVQFCL TIPPSAPPIA PPGTPAVVLK SKMLFFLQLT QKTSVPPQEP
  1381  VSIIVPIIYD MASGTTQQAD IPRQQNSSVA APMMVSNILK RFAEMNPPRQ GECTIFAAVR
  1441  DLMANLTLPP GGRP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MED14 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.38
Highest tissue expression
57 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 57 nTPM
  • tongue: 31 nTPM
  • thymus: 23 nTPM
  • bone marrow: 21 nTPM
  • epididymis: 19 nTPM
  • retina: 18 nTPM

Single-cell type

  • myonuclei: 212 nCPM
  • pituicytes/fscs: 164 nCPM
  • thymocytes: 106 nCPM
  • oligodendrocytes: 96 nCPM
  • gonadotrophs: 92 nCPM
  • neutrophils: 89 nCPM

Immune cell

  • NK-cell: 3.5 nTPM
  • intermediate monocyte: 3.2 nTPM
  • eosinophil: 2.9 nTPM
  • myeloid DC: 2.6 nTPM
  • MAIT T-cell: 2.2 nTPM
  • naive CD4 T-cell: 2 nTPM

Brain region

  • white matter: 28 nTPM
  • choroid plexus: 28 nTPM
  • thalamus: 26 nTPM
  • cerebral cortex: 25 nTPM
  • pons: 24 nTPM
  • basal ganglia: 24 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.09
gnomAD pLI
1
gnomAD missense Z
4.13
DepMap mean gene effect
-1.49
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Mediator complex, subunit Med14
  • Mediator of RNA polymerase II transcription subunit 14, RM8
  • Mediator of RNA polymerase II transcription subunit 14, RM2
  • Mediator of RNA polymerase II transcription subunit 14, RM6
  • Mediator complex subunit MED14, N-terminal
  • Mediator of RNA polymerase II transcription subunit 14, C-terminal domain
  • Mediator of RNA polymerase II transcription subunit 14, RM5
  • Mediator of RNA polymerase II transcription subunit 14, RM3
  • Mediator complex subunit MED14
  • Mediator of RNA polymerase II transcription subunit 14, RM2
  • Mediator of RNA polymerase II transcription subunit 14, RM8
  • Mediator of RNA polymerase II transcription subunit 14, RM6
  • Med14 RM domain
  • Med14 RM domain
  • Med14 C-terminal domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MED14 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MED14 as an antibody target. Whether an autoantibody or antibody against MED14 could matter depends on whether native MED14 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MED14 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MED14 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MED14. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...