GTF2F2
General transcription factor IIF subunit 2
Also known as: BTF4, RAP30, T2FB_HUMAN, TFIIF
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P13984
- Gene
- GTF2F2
- Ensembl
- ENSG00000188342
- Chromosome
- 13
- Canonical length
- 249 aa
- Protein class
- Predicted intracellular proteins
- Subcellular location
- Nucleoplasm,Microtubules
OverviewNCBI Gene
Predicted to enable RNA polymerase II general transcription initiation factor activity. Involved in positive regulation of transcription by RNA polymerase II; transcription elongation by RNA polymerase II; and transcription initiation at RNA polymerase II promoter. Located in microtubule cytoskeleton and nucleoplasm. Part of transcription factor TFIIF complex. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
249 residues, UniProt reviewed canonical sequence.
>P13984|GTF2F2
1 MAERGELDLT GAKQNTGVWL VKVPKYLSQQ WAKASGRGEV GKLRIAKTQG RTEVSFTLNE
61 DLANIHDIGG KPASVSAPRE HPFVLQSVGG QTLTVFTESS SDKLSLEGIV VQRAECRPAA
121 SENYMRLKRL QIEESSKPVR LSQQLDKVVT TNYKPVANHQ YNIEYERKKK EDGKRARADK
181 QHVLDMLFSA FEKHQYYNLK DLVDITKQPV VYLKEILKEI GVQNVKGIHK NTWELKPEYR
241 HYQGEEKSDLocalizationUniProt · AlphaFold · HPA
Whether an antibody against GTF2F2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.42
- Highest tissue expression
- 24 nTPM
Expression across tissuesHPA
Tissue
- skin: 24 nTPM
- skeletal muscle: 21 nTPM
- heart muscle: 19 nTPM
- colon: 17 nTPM
- midbrain: 17 nTPM
- adrenal gland: 16 nTPM
Single-cell type
- epididymal basal cells: 419 nCPM
- ocular epithelial cells: 389 nCPM
- endometrial glandular cells: 325 nCPM
- retinal pigment epithelial cells: 260 nCPM
- choroid plexus epithelial cells: 252 nCPM
- neutrophils: 250 nCPM
Immune cell
- eosinophil: 28 nTPM
- neutrophil: 21 nTPM
- myeloid DC: 18 nTPM
- non-classical monocyte: 17 nTPM
- NK-cell: 17 nTPM
- naive B-cell: 17 nTPM
Brain region
- choroid plexus: 18 nTPM
- white matter: 12 nTPM
- spinal cord: 9.5 nTPM
- hippocampal formation: 9.1 nTPM
- basal ganglia: 9 nTPM
- hypothalamus: 8.8 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.85
- gnomAD pLI
- 0
- gnomAD missense Z
- 1.29
- DepMap mean gene effect
- -0.83
- DepMap dependency class
- common
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- positive regulation of transcription by RNA polymerase II
- transcription by RNA polymerase II
- transcription elongation by RNA polymerase II
- transcription initiation at RNA polymerase II promoter
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Transcription Factor IIF, Rap30/Rap74, interaction
- Winged helix-like DNA-binding domain superfamily
- Winged helix DNA-binding domain superfamily
- Transcription initiation factor IIF, beta subunit
- TFIIF beta subunit, HTH domain
- TFIIF, beta subunit, N-terminal
- TFIIF, beta subunit HTH domain
- TFIIF, beta subunit N-terminus
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of GTF2F2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads GTF2F2 as an antibody target. Whether an autoantibody or antibody against GTF2F2 could matter depends on whether native GTF2F2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
GTF2F2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label GTF2F2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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