Seroatlas · Human Serome Atlas

POLR1E

DNA-directed RNA polymerase I subunit RPA49

Also known as: FLJ13390, FLJ13970, PAF53, PRAF1, RPA49, RPA49_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9GZS1
Gene: POLR1E
Ensembl: ENSG00000137054
Chromosome: 9
Canonical length: 419 aa
Protein class: Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins, RNA polymerase related proteins
Subcellular location: Nucleoplasm,Nucleoli fibrillar center

OverviewNCBI Gene

Predicted to enable DNA binding activity and RNA polymerase I general transcription initiation factor binding activity. Involved in nucleolar large rRNA transcription by RNA polymerase I and transcription initiation at RNA polymerase I promoter. Located in fibrillar center and nucleoplasm. Part of RNA polymerase I complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

419 residues, UniProt reviewed canonical sequence.

>Q9GZS1|POLR1E
     1  MAAEVLPSAR WQYCGAPDGS QRAVLVQFSN GKLQSPGNMR FTLYENKDST NPRKRNQRIL
    61  AAETDRLSYV GNNFGTGALK CNTLCRHFVG ILNKTSGQME VYDAELFNMQ PLFSDVSVES
   121  ELALESQTKT YREKMDSCIE AFGTTKQKRA LNTRRMNRVG NESLNRAVAK AAETIIDTKG
   181  VTALVSDAIH NDLQDDSLYL PPCYDDAAKP EDVYKFEDLL SPAEYEALQS PSEAFRNVTS
   241  EEILKMIEEN SHCTFVIEAL KSLPSDVESR DRQARCIWFL DTLIKFRAHR VVKRKSALGP
   301  GVPHIINTKL LKHFTCLTYN NGRLRNLISD SMKAKITAYV IILALHIHDF QIDLTVLQRD
   361  LKLSEKRMME IAKAMRLKIS KRRVSVAAGS EEDHKLGTLS LPLPPAQTSD RLAKRRKIT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POLR1E can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.37
Highest tissue expression: 34 nTPM

Expression across tissuesHPA

Tissue

ovary: 34 nTPM
liver: 28 nTPM
pancreas: 27 nTPM
endometrium: 23 nTPM
breast: 22 nTPM
cervix: 21 nTPM

Single-cell type

decidual stromal cells: 54 nCPM
plasma cells: 50 nCPM
pancreatic acinar cells: 39 nCPM
smooth muscle cells: 37 nCPM
esophageal basal cells: 34 nCPM
t-cells: 34 nCPM

Immune cell

MAIT T-cell: 23 nTPM
naive CD4 T-cell: 17 nTPM
naive CD8 T-cell: 15 nTPM
memory CD8 T-cell: 15 nTPM
gdT-cell: 14 nTPM
memory CD4 T-cell: 14 nTPM

Brain region

hypothalamus: 17 nTPM
spinal cord: 16 nTPM
white matter: 16 nTPM
thalamus: 15 nTPM
basal ganglia: 15 nTPM
midbrain: 15 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.92
gnomAD pLI: 0
gnomAD missense Z: 0.74
DepMap mean gene effect: -0.69
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

DNA binding
RNA polymerase I general transcription initiation factor binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

RNA polymerase I associated factor, A49-like
A49-like RNA polymerase I associated factor

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of POLR1E in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POLR1E as an antibody target. Whether an autoantibody or antibody against POLR1E could matter depends on whether native POLR1E is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POLR1E is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POLR1E as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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