Seroatlas · Human Serome Atlas

POLR2B

DNA-directed RNA polymerase II subunit RPB2

Also known as: RPB2, RPB2_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P30876
Gene: POLR2B
Ensembl: ENSG00000047315
Chromosome: 4
Canonical length: 1174 aa
Protein class: Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins, RNA polymerase related proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Canonical amino-acid sequenceUniProt

1174 residues, UniProt reviewed canonical sequence.

>P30876|POLR2B
     1  MYDADEDMQY DEDDDEITPD LWQEACWIVI SSYFDEKGLV RQQLDSFDEF IQMSVQRIVE
    61  DAPPIDLQAE AQHASGEVEE PPRYLLKFEQ IYLSKPTHWE RDGAPSPMMP NEARLRNLTY
   121  SAPLYVDITK TVIKEGEEQL QTQHQKTFIG KIPIMLRSTY CLLNGLTDRD LCELNECPLD
   181  PGGYFIINGS EKVLIAQEKM ATNTVYVFAK KDSKYAYTGE CRSCLENSSR PTSTIWVSML
   241  ARGGQGAKKS AIGQRIVATL PYIKQEVPII IVFRALGFVS DRDILEHIIY DFEDPEMMEM
   301  VKPSLDEAFV IQEQNVALNF IGSRGAKPGV TKEKRIKYAK EVLQKEMLPH VGVSDFCETK
   361  KAYFLGYMVH RLLLAALGRR ELDDRDHYGN KRLDLAGPLL AFLFRGMFKN LLKEVRIYAQ
   421  KFIDRGKDFN LELAIKTRII SDGLKYSLAT GNWGDQKKAH QARAGVSQVL NRLTFASTLS
   481  HLRRLNSPIG RDGKLAKPRQ LHNTLWGMVC PAETPEGHAV GLVKNLALMA YISVGSQPSP
   541  ILEFLEEWSM ENLEEISPAA IADATKIFVN GCWVGIHKDP EQLMNTLRKL RRQMDIIVSE
   601  VSMIRDIRER EIRIYTDAGR ICRPLLIVEK QKLLLKKRHI DQLKEREYNN YSWQDLVASG
   661  VVEYIDTLEE ETVMLAMTPD DLQEKEVAYC STYTHCEIHP SMILGVCASI IPFPDHNQSP
   721  RNTYQSAMGK QAMGVYITNF HVRMDTLAHV LYYPQKPLVT TRSMEYLRFR ELPAGINSIV
   781  AIASYTGYNQ EDSVIMNRSA VDRGFFRSVF YRSYKEQESK KGFDQEEVFE KPTRETCQGM
   841  RHAIYDKLDD DGLIAPGVRV SGDDVIIGKT VTLPENEDEL ESTNRRYTKR DCSTFLRTSE
   901  TGIVDQVMVT LNQEGYKFCK IRVRSVRIPQ IGDKFASRHG QKGTCGIQYR QEDMPFTCEG
   961  ITPDIIINPH AIPSRMTIGH LIECLQGKVS ANKGEIGDAT PFNDAVNVQK ISNLLSDYGY
  1021  HLRGNEVLYN GFTGRKITSQ IFIGPTYYQR LKHMVDDKIH SRARGPIQIL NRQPMEGRSR
  1081  DGGLRFGEME RDCQIAHGAA QFLRERLFEA SDPYQVHVCN LCGIMAIANT RTHTYECRGC
  1141  RNKTQISLVR MPYACKLLFQ ELMSMSIAPR MMSV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POLR2B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.25
Highest tissue expression: 61 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 61 nTPM
thymus: 57 nTPM
tongue: 53 nTPM
tonsil: 48 nTPM
parathyroid gland: 47 nTPM
lymph node: 47 nTPM

Single-cell type

cardiomyocytes: 145 nCPM
endometrial ciliated cells: 115 nCPM
pericytes: 112 nCPM
vascular smooth muscle cells: 109 nCPM
nk-cells: 106 nCPM
pancreatic acinar cells: 106 nCPM

Immune cell

basophil: 52 nTPM
T-reg: 31 nTPM
naive CD8 T-cell: 31 nTPM
MAIT T-cell: 30 nTPM
memory CD8 T-cell: 29 nTPM
memory B-cell: 28 nTPM

Brain region

cerebellum: 52 nTPM
choroid plexus: 44 nTPM
white matter: 44 nTPM
hypothalamus: 42 nTPM
pons: 40 nTPM
cerebral cortex: 38 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about POLR2B.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 80 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Esophageal atresia/tracheoesophageal fistula

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.34
gnomAD pLI: 0.52
gnomAD missense Z: 6.62
DepMap mean gene effect: -2.42
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

transcription by RNA polymerase II

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of POLR2B in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POLR2B as an antibody target. Whether an autoantibody or antibody against POLR2B could matter depends on whether native POLR2B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POLR2B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POLR2B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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