Seroatlas · Human Serome Atlas

MED4

Mediator of RNA polymerase II transcription subunit 4

Also known as: DRIP36, HSPC126, MED4_HUMAN, TRAP36, VDRIP

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NPJ6
Gene: MED4
Ensembl: ENSG00000136146
Chromosome: 13
Canonical length: 270 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Canonical amino-acid sequenceUniProt

270 residues, UniProt reviewed canonical sequence.

>Q9NPJ6|MED4
     1  MAASSSGEKE KERLGGGLGV AGGNSTRERL LSALEDLEVL SRELIEMLAI SRNQKLLQAG
    61  EENQVLELLI HRDGEFQELM KLALNQGKIH HEMQVLEKEV EKRDSDIQQL QKQLKEAEQI
   121  LATAVYQAKE KLKSIEKARK GAISSEEIIK YAHRISASNA VCAPLTWVPG DPRRPYPTDL
   181  EMRSGLLGQM NNPSTNGVNG HLPGDALAAG RLPDVLAPQY PWQSNDMSMN MLPPNHSSDF
   241  LLEPPGHNKE NEDDVEIMST DSSSSSSESD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MED4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.56
Highest tissue expression: 74 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 74 nTPM
thymus: 59 nTPM
tongue: 55 nTPM
skeletal muscle: 54 nTPM
testis: 52 nTPM
tonsil: 52 nTPM

Single-cell type

late spermatids: 270 nCPM
differentiating spermatogonia: 207 nCPM
syncytiotrophoblasts: 178 nCPM
late primary spermatocytes: 168 nCPM
megakaryocytes: 167 nCPM
esophageal apical cells: 164 nCPM

Immune cell

eosinophil: 107 nTPM
basophil: 106 nTPM
T-reg: 102 nTPM
total PBMC: 91 nTPM
NK-cell: 87 nTPM
memory CD4 T-cell: 85 nTPM

Brain region

cerebellum: 43 nTPM
white matter: 32 nTPM
cerebral cortex: 30 nTPM
pons: 30 nTPM
hypothalamus: 29 nTPM
amygdala: 29 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.79
gnomAD pLI: 0.01
gnomAD missense Z: 1.02
DepMap mean gene effect: -1.28
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Mediator complex, subunit Med4
Vitamin-D-receptor interacting Mediator subunit 4

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MED4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MED4 as an antibody target. Whether an autoantibody or antibody against MED4 could matter depends on whether native MED4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MED4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MED4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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