Seroatlas · Human Serome Atlas

RPS25

Small ribosomal subunit protein eS25

Also known as: RS25_HUMAN, S25

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P62851
Gene
RPS25
Ensembl
ENSG00000118181
Chromosome
11
Canonical length
125 aa
Protein class
Predicted intracellular proteins, Ribosomal proteins
Subcellular location
Nucleoli,Endoplasmic reticulum,Cytosol

OverviewNCBI Gene

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S25E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

125 residues, UniProt reviewed canonical sequence.

>P62851|RPS25
     1  MPPKDDKKKK DAGKSAKKDK DPVNKSGGKA KKKKWSKGKV RDKLNNLVLF DKATYDKLCK
    61  EVPNYKLITP AVVSERLKIR GSLARAALQE LLSKGLIKLV SKHRAQVIYT RNTKGGDAPA
   121  AGEDA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPS25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.54
Highest tissue expression
4,962 nTPM

Expression across tissuesHPA

Tissue

  • ovary: 4,962 nTPM
  • skeletal muscle: 3,801 nTPM
  • pancreas: 3,118 nTPM
  • cervix: 2,612 nTPM
  • skin: 2,565 nTPM
  • blood vessel: 2,483 nTPM

Single-cell type

  • epididymal efferent duct absorptive cells: 3,611 nCPM
  • ovarian stromal cells: 3,254 nCPM
  • gastric chief cells: 3,154 nCPM
  • enteric stem cells: 3,021 nCPM
  • epididymal basal cells: 2,715 nCPM
  • granulosa cells: 2,537 nCPM

Immune cell

  • total PBMC: 5,200 nTPM
  • naive CD4 T-cell: 4,522 nTPM
  • memory B-cell: 3,732 nTPM
  • naive CD8 T-cell: 3,675 nTPM
  • memory CD4 T-cell: 3,667 nTPM
  • MAIT T-cell: 3,228 nTPM

Brain region

  • spinal cord: 340 nTPM
  • white matter: 325 nTPM
  • hypothalamus: 302 nTPM
  • basal ganglia: 288 nTPM
  • medulla oblongata: 286 nTPM
  • thalamus: 286 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.46
gnomAD pLI
0.86
gnomAD missense Z
1.83
DepMap mean gene effect
-1.31
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPS25 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPS25 as an antibody target. Whether an autoantibody or antibody against RPS25 could matter depends on whether native RPS25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPS25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPS25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RPS25. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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