Seroatlas · Human Serome Atlas

RPS28

Small ribosomal subunit protein eS28

Also known as: eS28, RS28_HUMAN, S28

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P62857
Gene
RPS28
Ensembl
ENSG00000233927
Chromosome
19
Canonical length
69 aa
Protein class
Disease related genes, Human disease related genes, Predicted intracellular proteins, Ribosomal proteins
Subcellular location
Cytosol

OverviewNCBI Gene

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S28E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

69 residues, UniProt reviewed canonical sequence.

>P62857|RPS28
     1  MDTSRVQPIK LARVTKVLGR TGSQGQCTQV RVEFMDDTSR SIIRNVKGPV REGDVLTLLE
    61  SEREARRLR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPS28 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.4
Highest tissue expression
421 nTPM

Expression across tissuesHPA

Tissue

  • pancreas: 421 nTPM
  • skin: 339 nTPM
  • esophagus: 322 nTPM
  • ovary: 305 nTPM
  • bone marrow: 271 nTPM
  • spleen: 263 nTPM

Single-cell type

  • gastric chief cells: 9,109 nCPM
  • breast secretory cells: 7,410 nCPM
  • parietal cells: 7,047 nCPM
  • suprabasal keratinocytes: 6,634 nCPM
  • enteric stem cells: 6,268 nCPM
  • enteric transient amplifying cells: 5,665 nCPM

Immune cell

  • total PBMC: 166 nTPM
  • memory B-cell: 105 nTPM
  • naive CD4 T-cell: 99 nTPM
  • plasmacytoid DC: 98 nTPM
  • naive B-cell: 91 nTPM
  • memory CD4 T-cell: 89 nTPM

Brain region

  • thalamus: 75 nTPM
  • spinal cord: 65 nTPM
  • basal ganglia: 64 nTPM
  • medulla oblongata: 60 nTPM
  • white matter: 57 nTPM
  • choroid plexus: 56 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RPS28.

Disease | AllUniProt

Conditions RPS28 is implicated in, by any mechanism.

  • Diamond-Blackfan anemia 15, with mandibulofacial dysostosis (DBA15) MIM:606164

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.8
gnomAD pLI
0.68
gnomAD missense Z
1.51
DepMap mean gene effect
-1.81
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPS28 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPS28 as an antibody target. Whether an autoantibody or antibody against RPS28 could matter depends on whether native RPS28 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPS28 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPS28 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RPS28. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...