RACK1
Small ribosomal subunit protein RACK1
Also known as: Gnb2-rs1, GNB2L1, H12.3, RACK1_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P63244
- Gene
- RACK1
- Ensembl
- ENSG00000204628
- Chromosome
- 5
- Canonical length
- 317 aa
- Protein class
- Plasma proteins, Predicted intracellular proteins, Ribosomal proteins, Transporters
- Subcellular location
- Nucleoplasm,Cytosol
- Quaternary structure
- Homodimer
OverviewNCBI Gene
Enables several functions, including cyclin binding activity; enzyme binding activity; and protein domain specific binding activity. Involved in several processes, including regulation of macromolecule metabolic process; regulation of signal transduction; and regulation of vesicle-mediated transport. Located in several cellular components, including midbody; perinuclear region of cytoplasm; and phagocytic cup. Part of IRE1-RACK1-PP2A complex. [provided by Alliance of Genome Resources, Apr 2025]
Canonical amino-acid sequenceUniProt
317 residues, UniProt reviewed canonical sequence.
>P63244|RACK1
1 MTEQMTLRGT LKGHNGWVTQ IATTPQFPDM ILSASRDKTI IMWKLTRDET NYGIPQRALR
61 GHSHFVSDVV ISSDGQFALS GSWDGTLRLW DLTTGTTTRR FVGHTKDVLS VAFSSDNRQI
121 VSGSRDKTIK LWNTLGVCKY TVQDESHSEW VSCVRFSPNS SNPIIVSCGW DKLVKVWNLA
181 NCKLKTNHIG HTGYLNTVTV SPDGSLCASG GKDGQAMLWD LNEGKHLYTL DGGDIINALC
241 FSPNRYWLCA ATGPSIKIWD LEGKIIVDEL KQEVISTSSK AEPPQCTSLA WSADGQTLFA
301 GYTDNLVRVW QVTIGTRLocalizationUniProt · AlphaFold · HPA
Whether an antibody against RACK1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Cell surface
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.22
- Highest tissue expression
- 2,407 nTPM
Expression across tissuesHPA
Tissue
- ovary: 2,407 nTPM
- pancreas: 1,942 nTPM
- thymus: 1,586 nTPM
- skin: 1,507 nTPM
- bone marrow: 1,500 nTPM
- tonsil: 1,489 nTPM
Single-cell type
- decidual stromal cells: 5,853 nCPM
- esophageal basal cells: 5,534 nCPM
- esophageal suprabasal cells: 5,493 nCPM
- extravillous trophoblasts: 5,479 nCPM
- migrating cytotrophoblasts: 4,510 nCPM
- pancreatic acinar cells: 4,058 nCPM
Immune cell
- total PBMC: 3,380 nTPM
- memory B-cell: 1,739 nTPM
- naive B-cell: 1,696 nTPM
- naive CD4 T-cell: 1,603 nTPM
- T-reg: 1,469 nTPM
- myeloid DC: 1,408 nTPM
Brain region
- white matter: 377 nTPM
- medulla oblongata: 359 nTPM
- spinal cord: 344 nTPM
- choroid plexus: 320 nTPM
- thalamus: 294 nTPM
- hypothalamus: 287 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.16
- gnomAD pLI
- 1
- DepMap mean gene effect
- -1.86
- DepMap dependency class
- pan
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- cellular response to glucose stimulus
- cellular response to growth factor stimulus
- cytoplasmic translation
- gastrulation
- negative regulation of cell growth
- negative regulation of endoplasmic reticulum unfolded protein response
- negative regulation of gene expression
- negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide
- negative regulation of phagocytosis
- negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
- negative regulation of protein binding
- negative regulation of smoothened signaling pathway
- negative regulation of translation
- negative regulation of translational frameshifting
- negative regulation of Wnt signaling pathway
- pigmentation
- positive regulation of apoptotic process
- positive regulation of cell migration
- positive regulation of gastrulation
- positive regulation of Golgi to plasma membrane protein transport
- positive regulation of GTPase activity
- positive regulation of intrinsic apoptotic signaling pathway
- positive regulation of mitochondrial depolarization
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process
- positive regulation of protein phosphorylation
- positive regulation of protein-containing complex assembly
- protein ubiquitination
- regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway
- regulation of cell cycle
- regulation of cell division
- regulation of establishment of cell polarity
- regulation of protein localization
- rescue of stalled ribosome
- rhythmic process
Molecular functions
- BH3 domain binding
- cadherin binding
- cyclin binding
- cysteine-type endopeptidase activator activity involved in apoptotic process
- enzyme activator activity
- enzyme binding
- identical protein binding
- ion channel inhibitor activity
- molecular adaptor activity
- protein homodimerization activity
- protein kinase C binding
- protein phosphatase binding
- protein serine/threonine kinase inhibitor activity
- protein tyrosine kinase inhibitor activity
- receptor tyrosine kinase binding
- ribosome binding
- RNA binding
- SH2 domain binding
- signaling adaptor activity
- signaling receptor binding
- translation regulator activity
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of RACK1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads RACK1 as an antibody target. Whether an autoantibody or antibody against RACK1 could matter depends on whether native RACK1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
RACK1 is annotated at the cell surface, where native RACK1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.
Annotation status
The present source text does not explicitly label RACK1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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