Seroatlas · Human Serome Atlas

RPS3

Small ribosomal subunit protein uS3

Also known as: FLJ26283, FLJ27450, MGC87870, RS3_HUMAN, S3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P23396
Gene
RPS3
Ensembl
ENSG00000149273
Chromosome
11
Canonical length
243 aa
Protein class
Cancer-related genes, Enzymes, Plasma proteins, Predicted intracellular proteins, Ribosomal proteins
Subcellular location
Endoplasmic reticulum,Cytosol

OverviewNCBI Gene

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Canonical amino-acid sequenceUniProt

243 residues, UniProt reviewed canonical sequence.

>P23396|RPS3
     1  MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG
    61  EKGRRIRELT AVVQKRFGFP EGSVELYAEK VATRGLCAIA QAESLRYKLL GGLAVRRACY
   121  GVLRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG
   181  VLGIKVKIML PWDPTGKIGP KKPLPDHVSI VEPKDEILPT TPISEQKGGK PEPPAMPQPV
   241  PTA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPS3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.39
Highest tissue expression
2,900 nTPM

Expression across tissuesHPA

Tissue

  • ovary: 2,900 nTPM
  • bone marrow: 1,960 nTPM
  • breast: 1,821 nTPM
  • pancreas: 1,677 nTPM
  • skin: 1,671 nTPM
  • esophagus: 1,565 nTPM

Single-cell type

  • esophageal basal cells: 11,757 nCPM
  • esophageal suprabasal cells: 11,421 nCPM
  • extravillous trophoblasts: 10,717 nCPM
  • decidual stromal cells: 10,162 nCPM
  • migrating cytotrophoblasts: 9,812 nCPM
  • gastric progenitor cells: 8,096 nCPM

Immune cell

  • total PBMC: 4,239 nTPM
  • naive CD4 T-cell: 2,108 nTPM
  • MAIT T-cell: 1,928 nTPM
  • memory CD4 T-cell: 1,828 nTPM
  • naive CD8 T-cell: 1,639 nTPM
  • memory B-cell: 1,608 nTPM

Brain region

  • medulla oblongata: 201 nTPM
  • spinal cord: 199 nTPM
  • basal ganglia: 197 nTPM
  • white matter: 194 nTPM
  • hypothalamus: 186 nTPM
  • cerebral cortex: 180 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.38
gnomAD pLI
0.92
gnomAD missense Z
2.78
DepMap mean gene effect
-2.47
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPS3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPS3 as an antibody target. Whether an autoantibody or antibody against RPS3 could matter depends on whether native RPS3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPS3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPS3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RPS3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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