NELFA

Protein identityUniProt · HPA

UniProt accession

Q9H3P2

Gene

NELFA

Ensembl

ENSG00000185049

Chromosome

4

Canonical length

528 aa

Protein class

Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nuclear bodies

OverviewNCBI Gene

This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

528 residues, UniProt reviewed canonical sequence.

>Q9H3P2|NELFA
     1  MASMRESDTG LWLHNKLGAT DELWAPPSIA SLLTAAVIDN IRLCFHGLSS AVKLKLLLGT
    61  LHLPRRTVDE MKGALMEIIQ LASLDSDPWV LMVADILKSF PDTGSLNLEL EEQNPNVQDI
   121  LGELREKVGE CEASAMLPLE CQYLNKNALT TLAGPLTPPV KHFQLKRKPK SATLRAELLQ
   181  KSTETAQQLK RSAGVPFHAK GRGLLRKMDT TTPLKGIPKQ APFRSPTAPS VFSPTGNRTP
   241  IPPSRTLLRK ERGVKLLDIS ELDMVGAGRE AKRRRKTLDA EVVEKPAKEE TVVENATPDY
   301  AAGLVSTQKL GSLNNEPALP STSYLPSTPS VVPASSYIPS SETPPAPSSR EASRPPEEPS
   361  APSPTLPAQF KQRAPMYNSG LSPATPTPAA PTSPLTPTTP PAVAPTTQTP PVAMVAPQTQ
   421  APAQQQPKKN LSLTREQMFA AQEMFKTANK VTRPEKALIL GFMAGSRENP CQEQGDVIQI
   481  KLSEHTEDLP KADGQGSTTM LVDTVFEMNY ATGQWTRFKK YKPMTNVS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NELFA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.54

Highest tissue expression

28 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NELFA.

Disease | ImmuneIEDB

Conditions an epitope on NELFA was assayed in.

• castration-resistant prostate carcinoma B and T cell
• prostate cancer B and T cell
• prostate adenocarcinoma B and T cell
• biliary tract cancer B and T cell
• esophageal cancer B and T cell
• carcinoma B and T cell
• triple-receptor negative breast cancer B and T cell
• Her2-receptor negative breast cancer B and T cell
• Her2-receptor positive breast cancer B and T cell
• colorectal cancer B and T cell
• synovial sarcoma B and T cell
• leiomyosarcoma B and T cell
• lung cancer B and T cell
• prostatic urethral cancer B and T cell
• cancer B cell
• pancreatic carcinoma B cell
• lung small cell carcinoma B cell
• adult hepatocellular carcinoma B cell
• hematologic cancer B cell
• hepatocellular carcinoma B cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.51

gnomAD pLI

0.13

gnomAD missense Z

1.82

DepMap mean gene effect

-0.91

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• negative regulation of transcription elongation by RNA polymerase II
• positive regulation of transcription by RNA polymerase II

Molecular functions

• chromatin binding
• molecular adaptor activity

Cellular components

• cytosol
• NELF complex
• nuclear body
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Hepatitis delta antigen (HDAg) domain
• Negative elongation factor A domain-containing protein
• NELF-A, N-terminal domain
• NELF-A N-terminal domain

KeywordsUniProt

• Nucleus
• Phosphoprotein
• Repressor
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of NELFA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• NELFB
• NELFCD
• NELFE
• OPRM1
• POLR2B
• SUPT5H