POLR2H
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Also known as: RPAB3_HUMAN, RPB8
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P52434
- Gene
- POLR2H
- Ensembl
- ENSG00000163882
- Chromosome
- 3
- Canonical length
- 150 aa
- Protein class
- Metabolic proteins, Predicted intracellular proteins, RNA polymerase related proteins
- Subcellular location
- Nucleoplasm
OverviewNCBI Gene
The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
Canonical amino-acid sequenceUniProt
150 residues, UniProt reviewed canonical sequence.
>P52434|POLR2H
1 MAGILFEDIF DVKDIDPEGK KFDRVSRLHC ESESFKMDLI LDVNIQIYPV DLGDKFRLVI
61 ASTLYEDGTL DDGEYNPTDD RPSRADQFEY VMYGKVYRIE GDETSTEAAT RLSAYVSYGG
121 LLMRLQGDAN NLHGFEVDSR VYLLMKKLAFLocalizationUniProt · AlphaFold · HPA
Whether an antibody against POLR2H can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.31
- Highest tissue expression
- 76 nTPM
Expression across tissuesHPA
Tissue
- kidney: 76 nTPM
- liver: 62 nTPM
- choroid plexus: 60 nTPM
- adrenal gland: 60 nTPM
- duodenum: 54 nTPM
- rectum: 54 nTPM
Single-cell type
- gastric progenitor cells: 228 nCPM
- esophageal basal cells: 177 nCPM
- extravillous trophoblasts: 169 nCPM
- migrating cytotrophoblasts: 163 nCPM
- megakaryocytes: 136 nCPM
- syncytiotrophoblasts: 131 nCPM
Immune cell
- naive CD4 T-cell: 46 nTPM
- T-reg: 37 nTPM
- plasmacytoid DC: 35 nTPM
- naive CD8 T-cell: 35 nTPM
- naive B-cell: 34 nTPM
- myeloid DC: 34 nTPM
Brain region
- cerebellum: 25 nTPM
- white matter: 24 nTPM
- cerebral cortex: 22 nTPM
- choroid plexus: 22 nTPM
- thalamus: 21 nTPM
- basal ganglia: 20 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.62
- gnomAD pLI
- 0.65
- gnomAD missense Z
- 1.57
- DepMap mean gene effect
- -1.64
- DepMap dependency class
- pan
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Nucleic acid-binding, OB-fold
- DNA-directed RNA polymerases I, II, and III subunit RPABC3
- RNA polymerase Rpb8
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of POLR2H in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads POLR2H as an antibody target. Whether an autoantibody or antibody against POLR2H could matter depends on whether native POLR2H is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
POLR2H is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label POLR2H as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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