POLR2I
DNA-directed RNA polymerase II subunit RPB9
Also known as: hRPB14.5, RPB9, RPB9_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P36954
- Gene
- POLR2I
- Ensembl
- ENSG00000105258
- Chromosome
- 19
- Canonical length
- 125 aa
- Protein class
- Metabolic proteins, Predicted intracellular proteins, RNA polymerase related proteins
- Subcellular location
- Nucleoplasm
OverviewNCBI Gene
This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
125 residues, UniProt reviewed canonical sequence.
>P36954|POLR2I
1 MEPDGTYEPG FVGIRFCQEC NNMLYPKEDK ENRILLYACR NCDYQQEADN SCIYVNKITH
61 EVDELTQIIA DVSQDPTLPR TEDHPCQKCG HKEAVFFQSH SARAEDAMRL YYVCTAPHCG
121 HRWTELocalizationUniProt · AlphaFold · HPA
Whether an antibody against POLR2I can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.41
- Highest tissue expression
- 234 nTPM
Expression across tissuesHPA
Tissue
- skeletal muscle: 234 nTPM
- testis: 211 nTPM
- heart muscle: 178 nTPM
- spinal cord: 138 nTPM
- amygdala: 138 nTPM
- midbrain: 129 nTPM
Single-cell type
- late spermatids: 3,809 nCPM
- late primary spermatocytes: 1,113 nCPM
- early spermatids: 1,109 nCPM
- respiratory ciliated cells: 489 nCPM
- fallopian tube ciliated cells: 462 nCPM
- esophageal apical cells: 458 nCPM
Immune cell
- plasmacytoid DC: 225 nTPM
- T-reg: 177 nTPM
- memory B-cell: 170 nTPM
- naive CD4 T-cell: 151 nTPM
- memory CD4 T-cell: 149 nTPM
- naive B-cell: 136 nTPM
Brain region
- white matter: 115 nTPM
- cerebellum: 86 nTPM
- basal ganglia: 84 nTPM
- medulla oblongata: 78 nTPM
- hypothalamus: 77 nTPM
- cerebral cortex: 76 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.71
- gnomAD pLI
- 0.36
- gnomAD missense Z
- 1.49
- DepMap mean gene effect
- -1.66
- DepMap dependency class
- pan
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- transcription by RNA polymerase II
- transcription initiation at RNA polymerase II promoter
- transcription-coupled nucleotide-excision repair
- maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Zinc finger, TFIIS-type
- DNA-directed RNA polymerase II subunit RPB9-like, zinc ribbon
- DNA-directed RNA polymerase subunit/transcription factor S
- DNA-directed RNA polymerase M, 15kDa subunit, conserved site
- Transcription factor S-II (TFIIS)
- RNA polymerases M/15 Kd subunit
- Pol II subunit B9, C-terminal zinc ribbon
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of POLR2I in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads POLR2I as an antibody target. Whether an autoantibody or antibody against POLR2I could matter depends on whether native POLR2I is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
POLR2I is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label POLR2I as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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