Seroatlas · Human Serome Atlas

POLR2D

DNA-directed RNA polymerase II subunit RPB4

Also known as: RPB4, RPB4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O15514
Gene
POLR2D
Ensembl
ENSG00000144231
Chromosome
2
Canonical length
142 aa
Protein class
Metabolic proteins, Plasma proteins, Predicted intracellular proteins, RNA polymerase related proteins
Subcellular location
Nucleoplasm,Nuclear speckles,Cytosol

OverviewNCBI Gene

This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

142 residues, UniProt reviewed canonical sequence.

>O15514|POLR2D
     1  MAAGGSDPRA GDVEEDASQL IFPKEFETAE TLLNSEVHML LEHRKQQNES AEDEQELSEV
    61  FMKTLNYTAR FSRFKNRETI ASVRSLLLQK KLHKFELACL ANLCPETAEE SKALIPSLEG
   121  RFEDEELQQI LDDIQTKRSF QY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POLR2D can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
70 nTPM

Expression across tissuesHPA

Tissue

  • testis: 70 nTPM
  • bone marrow: 32 nTPM
  • thymus: 26 nTPM
  • skeletal muscle: 26 nTPM
  • tongue: 25 nTPM
  • tonsil: 25 nTPM

Single-cell type

  • late spermatids: 130 nCPM
  • late primary spermatocytes: 63 nCPM
  • differentiating spermatogonia: 42 nCPM
  • megakaryocyte progenitors: 35 nCPM
  • early primary spermatocytes: 35 nCPM
  • erythrocyte progenitors: 31 nCPM

Immune cell

  • non-classical monocyte: 35 nTPM
  • intermediate monocyte: 32 nTPM
  • myeloid DC: 30 nTPM
  • naive CD4 T-cell: 28 nTPM
  • gdT-cell: 26 nTPM
  • NK-cell: 26 nTPM

Brain region

  • white matter: 26 nTPM
  • basal ganglia: 24 nTPM
  • cerebellum: 24 nTPM
  • midbrain: 23 nTPM
  • hypothalamus: 22 nTPM
  • spinal cord: 22 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.9
gnomAD pLI
0.21
gnomAD missense Z
1.58
DepMap mean gene effect
-1.94
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of POLR2D in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POLR2D as an antibody target. Whether an autoantibody or antibody against POLR2D could matter depends on whether native POLR2D is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POLR2D is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POLR2D as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/POLR2D. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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