Seroatlas · Human Serome Atlas

POLR2F

DNA-directed RNA polymerases I, II, and III subunit RPABC2

Also known as: HRBP14.4, RPAB2_HUMAN, RPB6

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P61218
Gene
POLR2F
Ensembl
ENSG00000100142
Chromosome
22
Canonical length
127 aa
Protein class
Metabolic proteins, Predicted intracellular proteins, RNA polymerase related proteins
Subcellular location
Nucleoplasm,Nucleoli fibrillar center

OverviewNCBI Gene

This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Canonical amino-acid sequenceUniProt

127 residues, UniProt reviewed canonical sequence.

>P61218|POLR2F
     1  MSDNEDNFDG DDFDDVEEDE GLDDLENAEE EGQENVEILP SGERPQANQK RITTPYMTKY
    61  ERARVLGTRA LQIAMCAPVM VELEGETDPL LIAMKELKAR KIPIIIRRYL PDGSYEDWGV
   121  DELIITD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POLR2F can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.48
Highest tissue expression
112 nTPM

Expression across tissuesHPA

Tissue

  • liver: 112 nTPM
  • skeletal muscle: 106 nTPM
  • spinal cord: 96 nTPM
  • heart muscle: 87 nTPM
  • midbrain: 80 nTPM
  • choroid plexus: 77 nTPM

Single-cell type

  • oligodendrocytes: 628 nCPM
  • extravillous trophoblasts: 377 nCPM
  • early primary spermatocytes: 341 nCPM
  • hepatocytes: 332 nCPM
  • esophageal suprabasal cells: 330 nCPM
  • migrating cytotrophoblasts: 304 nCPM

Immune cell

  • eosinophil: 141 nTPM
  • neutrophil: 124 nTPM
  • plasmacytoid DC: 124 nTPM
  • basophil: 120 nTPM
  • NK-cell: 114 nTPM
  • T-reg: 104 nTPM

Brain region

  • white matter: 170 nTPM
  • cerebral cortex: 112 nTPM
  • basal ganglia: 110 nTPM
  • hypothalamus: 108 nTPM
  • medulla oblongata: 102 nTPM
  • thalamus: 99 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.88
gnomAD pLI
0.01
gnomAD missense Z
0.89
DepMap mean gene effect
-1.98
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • RNA polymerase, subunit omega/Rpo6/RPB6
  • Archaeal Rpo6/eukaryotic RPB6 RNA polymerase subunit
  • DNA-directed RNA polymerase, 14-18kDa subunit, conserved site
  • DNA-directed RNA polymerase, subunit RPB6
  • RPB6/omega subunit-like superfamily
  • RNA polymerase Rpb6

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of POLR2F in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POLR2F as an antibody target. Whether an autoantibody or antibody against POLR2F could matter depends on whether native POLR2F is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POLR2F is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POLR2F as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/POLR2F. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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