MED8
Mediator of RNA polymerase II transcription subunit 8
Also known as: ARC32, MED8_HUMAN, MGC17544, MGC19641
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q96G25
- Gene
- MED8
- Ensembl
- ENSG00000159479
- Chromosome
- 1
- Canonical length
- 268 aa
- Protein class
- Plasma proteins, Predicted intracellular proteins
- Subcellular location
- Nucleoplasm,Golgi apparatus
OverviewNCBI Gene
This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
Canonical amino-acid sequenceUniProt
268 residues, UniProt reviewed canonical sequence.
>Q96G25|MED8
1 MQREEKQLEA SLDALLSQVA DLKNSLGSFI CKLENEYGRL TWPSVLDSFA LLSGQLNTLN
61 KVLKHEKTPL FRNQVIIPLV LSPDRDEDLM RQTEGRVPVF SHEVVPDHLR TKPDPEVEEQ
121 EKQLTTDAAR IGADAAQKQI QSLNKMCSNL LEKISKEERE SESGGLRPNK QTFNPTDTNA
181 LVAAVAFGKG LSNWRPSGSS GPGQAGQPGA GTILAGTSGL QQVQMAGAPS QQQPMLSGVQ
241 MAQAGQPGKM PSGIKTNIKS ASMHPYQRLocalizationUniProt · AlphaFold · HPA
Whether an antibody against MED8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.54
- Highest tissue expression
- 40 nTPM
Expression across tissuesHPA
Tissue
- esophagus: 40 nTPM
- liver: 39 nTPM
- retina: 37 nTPM
- skin: 33 nTPM
- pituitary gland: 33 nTPM
- adipose tissue: 27 nTPM
Single-cell type
- esophageal apical cells: 233 nCPM
- oocytes: 143 nCPM
- esophageal suprabasal cells: 136 nCPM
- syncytiotrophoblasts: 109 nCPM
- extravillous trophoblasts: 93 nCPM
- breast lactating cells: 77 nCPM
Immune cell
- classical monocyte: 57 nTPM
- myeloid DC: 57 nTPM
- eosinophil: 55 nTPM
- intermediate monocyte: 53 nTPM
- non-classical monocyte: 49 nTPM
- neutrophil: 46 nTPM
Brain region
- choroid plexus: 26 nTPM
- medulla oblongata: 25 nTPM
- white matter: 25 nTPM
- pons: 24 nTPM
- thalamus: 22 nTPM
- midbrain: 21 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.1
- gnomAD pLI
- 0
- gnomAD missense Z
- 0.97
- DepMap mean gene effect
- -0.95
- DepMap dependency class
- common
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- positive regulation of transcription elongation by RNA polymerase II
- positive regulation of transcription initiation by RNA polymerase II
- protein ubiquitination
- regulation of transcription by RNA polymerase II
- RNA polymerase II preinitiation complex assembly
Molecular functions
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- transcription coregulator activity
- ubiquitin protein ligase activity
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Mediator complex, subunit Med8, fungi/metazoa
- Mediator of RNA polymerase II transcription complex subunit 8
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of MED8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads MED8 as an antibody target. Whether an autoantibody or antibody against MED8 could matter depends on whether native MED8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
MED8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label MED8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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