Seroatlas · Human Serome Atlas

MED16

Mediator of RNA polymerase II transcription subunit 16

Also known as: DRIP92, MED16_HUMAN, THRAP5, TRAP95

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y2X0
Gene: MED16
Ensembl: ENSG00000175221
Chromosome: 19
Canonical length: 877 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoli

OverviewNCBI Gene

Enables nuclear thyroid hormone receptor binding activity and transcription coactivator activity. Involved in positive regulation of transcription initiation by RNA polymerase II. Located in nucleus. Part of core mediator complex and mediator complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

877 residues, UniProt reviewed canonical sequence.

>Q9Y2X0|MED16
     1  MCDLRRPAAG GMMDLAYVCE WEKWSKSTHC PSVPLACAWS CRNLIAFTMD LRSDDQDLTR
    61  MIHILDTEHP WDLHSIPSEH HEAITCLEWD QSGSRLLSAD ADGQIKCWSM ADHLANSWES
   121  SVGSLVEGDP IVALSWLHNG VKLALHVEKS GASSFGEKFS RVKFSPSLTL FGGKPMEGWI
   181  AVTVSGLVTV SLLKPSGQVL TSTESLCRLR GRVALADIAF TGGGNIVVAT ADGSSASPVQ
   241  FYKVCVSVVS EKCRIDTEIL PSLFMRCTTD LNRKDKFPAI THLKFLARDM SEQVLLCASS
   301  QTSSIVECWS LRKEGLPVNN IFQQISPVVG DKQPTILKWR ILSATNDLDR VSAVALPKLP
   361  ISLTNTDLKV ASDTQFYPGL GLALAFHDGS VHIVHRLSLQ TMAVFYSSAA PRPVDEPAMK
   421  RPRTAGPAVH LKAMQLSWTS LALVGIDSHG KLSVLRLSPS MGHPLEVGLA LRHLLFLLEY
   481  CMVTGYDWWD ILLHVQPSMV QSLVEKLHEE YTRQTAALQQ VLSTRILAMK ASLCKLSPCT
   541  VTRVCDYHTK LFLIAISSTL KSLLRPHFLN TPDKSPGDRL TEICTKITDV DIDKVMINLK
   601  TEEFVLDMNT LQALQQLLQW VGDFVLYLLA SLPNQGSLLR PGHSFLRDGT SLGMLRELMV
   661  VIRIWGLLKP SCLPVYTATS DTQDSMSLLF RLLTKLWICC RDEGPASEPD EALVDECCLL
   721  PSQLLIPSLD WLPASDGLVS RLQPKQPLRL QFGRAPTLPG SAATLQLDGL ARAPGQPKID
   781  HLRRLHLGAC PTEECKACTR CGCVTMLKSP NRTTAVKQWE QRWIKNCLAV EGRGPDACVT
   841  SRASEEAPAF VQLGPQSTHH SPRTPRSLDH LHPEDRP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MED16 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.27
Highest tissue expression: 47 nTPM

Expression across tissuesHPA

Tissue

liver: 47 nTPM
pancreas: 44 nTPM
skeletal muscle: 36 nTPM
prostate: 34 nTPM
kidney: 34 nTPM
ovary: 32 nTPM

Single-cell type

oocytes: 92 nCPM
platelets: 70 nCPM
cytotrophoblasts: 57 nCPM
syncytiotrophoblasts: 55 nCPM
foveolar cells: 47 nCPM
migrating cytotrophoblasts: 42 nCPM

Immune cell

total PBMC: 37 nTPM
T-reg: 36 nTPM
memory CD8 T-cell: 30 nTPM
memory CD4 T-cell: 28 nTPM
gdT-cell: 28 nTPM
neutrophil: 26 nTPM

Brain region

cerebral cortex: 45 nTPM
basal ganglia: 42 nTPM
medulla oblongata: 42 nTPM
amygdala: 40 nTPM
thalamus: 39 nTPM
hippocampal formation: 38 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MED16.

Disease | AllUniProt

Conditions MED16 is implicated in, by any mechanism.

Guillouet-Gordon syndrome (GGNS) MIM:621220

Disease | GeneticClinVar

17 pathogenic / likely-pathogenic of 288 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Guillouet-Gordon syndrome
Syndromic intellectual disability

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.03
gnomAD pLI: 0
gnomAD missense Z: -1.67
DepMap mean gene effect: -0.16
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

WD40 repeat
Soluble quinoprotein glucose/sorbosone dehydrogenase, beta-propeller domain superfamily
WD40/YVTN repeat-like-containing domain superfamily
WD40-repeat-containing domain superfamily
WD domain, G-beta repeat
Mediator complex, subunit Med16, N-terminal
Mediator complex, subunit Med16
Mediator complex subunit 16, C-terminal
Mediator of RNA polymerase II transcription subunit 16 , central helical bridge
Mediator complex subunit 16, N-terminal
Mediator complex subunit 16, central helical bridge
Mediator complex subunit 16, C-terminal

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MED16 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MED16 as an antibody target. Whether an autoantibody or antibody against MED16 could matter depends on whether native MED16 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MED16 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MED16 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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