Seroatlas · Human Serome Atlas

MED20

Mediator of RNA polymerase II transcription subunit 20

Also known as: DKFZp586D2223, MED20_HUMAN, PRO0213, SRB2, TRFP

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9H944
Gene
MED20
Ensembl
ENSG00000124641
Chromosome
6
Canonical length
212 aa
Protein class
Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015]

Canonical amino-acid sequenceUniProt

212 residues, UniProt reviewed canonical sequence.

>Q9H944|MED20
     1  MGVTCVSQMP VAEGKSVQQT VELLTRKLEM LGAEKQGTFC VDCETYHTAA STLGSQGQTG
    61  KLMYVMHNSE YPLSCFALFE NGPCLIADTN FDVLMVKLKG FFQSAKASKI ETRGTRYQYC
   121  DFLVKVGTVT MGPSARGISV EVEYGPCVVA SDCWSLLLEF LQSFLGSHTP GAPAVFGNRH
   181  DAVYGPADTM VQYMELFNKI RKQQQVPVAG IR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MED20 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.3
Highest tissue expression
15 nTPM

Expression across tissuesHPA

Tissue

  • tongue: 15 nTPM
  • skeletal muscle: 13 nTPM
  • placenta: 13 nTPM
  • epididymis: 12 nTPM
  • tonsil: 12 nTPM
  • kidney: 12 nTPM

Single-cell type

  • distal convoluted tubule cells: 8.3 nCPM
  • choroid plexus epithelial cells: 5.7 nCPM
  • loop of henle epithelial cells: 5.7 nCPM
  • oligodendrocyte progenitor cells: 4.8 nCPM
  • renal collecting duct intercalated cells: 4.8 nCPM
  • ependymal cells: 4.6 nCPM

Immune cell

  • intermediate monocyte: 29 nTPM
  • myeloid DC: 26 nTPM
  • classical monocyte: 25 nTPM
  • naive B-cell: 23 nTPM
  • plasmacytoid DC: 23 nTPM
  • non-classical monocyte: 21 nTPM

Brain region

  • cerebellum: 18 nTPM
  • choroid plexus: 18 nTPM
  • white matter: 18 nTPM
  • thalamus: 17 nTPM
  • cerebral cortex: 16 nTPM
  • hypothalamus: 16 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.77
gnomAD pLI
0.3
gnomAD missense Z
1.23
DepMap mean gene effect
-1.36
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Mediator complex, subunit Med20
  • TATA-binding related factor (TRF) of subunit 20 of Mediator complex

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MED20 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MED20 as an antibody target. Whether an autoantibody or antibody against MED20 could matter depends on whether native MED20 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MED20 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MED20 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MED20. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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