Seroatlas · Human Serome Atlas

NDC80

Kinetochore protein NDC80 homolog

Also known as: HEC, HEC1, hsNDC80, KNTC2, NDC80_HUMAN, TID3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O14777
Gene
NDC80
Ensembl
ENSG00000080986
Chromosome
18
Canonical length
642 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nuclear speckles,Centrosome,Cytosol

OverviewNCBI Gene

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Canonical amino-acid sequenceUniProt

642 residues, UniProt reviewed canonical sequence.

>O14777|NDC80
     1  MKRSSVSSGG AGRLSMQELR SQDVNKQGLY TPQTKEKPTF GKLSINKPTS ERKVSLFGKR
    61  TSGHGSRNSQ LGIFSSSEKI KDPRPLNDKA FIQQCIRQLC EFLTENGYAH NVSMKSLQAP
   121  SVKDFLKIFT FLYGFLCPSY ELPDTKFEEE VPRIFKDLGY PFALSKSSMY TVGAPHTWPH
   181  IVAALVWLID CIKIHTAMKE SSPLFDDGQP WGEETEDGIM HNKLFLDYTI KCYESFMSGA
   241  DSFDEMNAEL QSKLKDLFNV DAFKLESLEA KNRALNEQIA RLEQEREKEP NRLESLRKLK
   301  ASLQGDVQKY QAYMSNLESH SAILDQKLNG LNEEIARVEL ECETIKQENT RLQNIIDNQK
   361  YSVADIERIN HERNELQQTI NKLTKDLEAE QQKLWNEELK YARGKEAIET QLAEYHKLAR
   421  KLKLIPKGAE NSKGYDFEIK FNPEAGANCL VKYRAQVYVP LKELLNETEE EINKALNKKM
   481  GLEDTLEQLN AMITESKRSV RTLKEEVQKL DDLYQQKIKE AEEEDEKCAS ELESLEKHKH
   541  LLESTVNQGL SEAMNELDAV QREYQLVVQT TTEERRKVGN NLQRLLEMVA THVGSVEKHL
   601  EEQIAKVDRE YEECMSEDLS ENIKEIRDKY EKKATLIKSS EE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NDC80 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.46
Highest tissue expression
29 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 29 nTPM
  • thymus: 27 nTPM
  • lymph node: 19 nTPM
  • tonsil: 18 nTPM
  • testis: 14 nTPM
  • seminal vesicle: 13 nTPM

Single-cell type

  • monocyte progenitors: 101 nCPM
  • oocytes: 83 nCPM
  • erythrocyte progenitors: 81 nCPM
  • late primary spermatocytes: 73 nCPM
  • early primary spermatocytes: 72 nCPM
  • megakaryocyte progenitors: 62 nCPM

Immune cell

  • T-reg: 11 nTPM
  • neutrophil: 8.4 nTPM
  • memory B-cell: 7.1 nTPM
  • naive B-cell: 5.3 nTPM
  • memory CD8 T-cell: 3.8 nTPM
  • basophil: 3.7 nTPM

Brain region

  • pons: 17 nTPM
  • cerebellum: 16 nTPM
  • white matter: 15 nTPM
  • medulla oblongata: 15 nTPM
  • cerebral cortex: 14 nTPM
  • basal ganglia: 14 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.7
gnomAD pLI
0
gnomAD missense Z
1.84
DepMap mean gene effect
-1.52
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Kinetochore protein Ndc80
  • Ndc80 domain superfamily
  • Domain of unknown function DUF5595
  • Kinetochore protein Ndc80, CH domain
  • Kinetochore protein NDC80 loop region
  • HEC/Ndc80p family
  • Domain of unknown function (DUF5595)
  • Kinetochore protein NDC80 loop region

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NDC80 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NDC80 as an antibody target. Whether an autoantibody or antibody against NDC80 could matter depends on whether native NDC80 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NDC80 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NDC80 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NDC80. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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