Seroatlas · Human Serome Atlas

MED15

Mediator of RNA polymerase II transcription subunit 15

Also known as: Arc105, CAG7A, MED15_HUMAN, PCQAP, TIG-1, TNRC7

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96RN5
Gene: MED15
Ensembl: ENSG00000099917
Chromosome: 22
Canonical length: 788 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Canonical amino-acid sequenceUniProt

788 residues, UniProt reviewed canonical sequence.

>Q96RN5|MED15
     1  MDVSGQETDW RSTAFRQKLV SQIEDAMRKA GVAHSKSSKD MESHVFLKAK TRDEYLSLVA
    61  RLIIHFRDIH NKKSQASVSD PMNALQSLTG GPAAGAAGIG MPPRGPGQSL GGMGSLGAMG
   121  QPMSLSGQPP PGTSGMAPHS MAVVSTATPQ TQLQLQQVAL QQQQQQQQFQ QQQQAALQQQ
   181  QQQQQQQQFQ AQQSAMQQQF QAVVQQQQQL QQQQQQQQHL IKLHHQNQQQ IQQQQQQLQR
   241  IAQLQLQQQQ QQQQQQQQQQ QQALQAQPPI QQPPMQQPQP PPSQALPQQL QQMHHTQHHQ
   301  PPPQPQQPPV AQNQPSQLPP QSQTQPLVSQ AQALPGQMLY TQPPLKFVRA PMVVQQPPVQ
   361  PQVQQQQTAV QTAQAAQMVA PGVQMITEAL AQGGMHIRAR FPPTTAVSAI PSSSIPLGRQ
   421  PMAQVSQSSL PMLSSPSPGQ QVQTPQSMPP PPQPSPQPGQ PSSQPNSNVS SGPAPSPSSF
   481  LPSPSPQPSQ SPVTARTPQN FSVPSPGPLN TPVNPSSVMS PAGSSQAEEQ QYLDKLKQLS
   541  KYIEPLRRMI NKIDKNEDRK KDLSKMKSLL DILTDPSKRC PLKTLQKCEI ALEKLKNDMA
   601  VPTPPPPPVP PTKQQYLCQP LLDAVLANIR SPVFNHSLYR TFVPAMTAIH GPPITAPVVC
   661  TRKRRLEDDE RQSIPSVLQG EVARLDPKFL VNLDPSHCSN NGTVHLICKL DDKDLPSVPP
   721  LELSVPADYP AQSPLWIDRQ WQYDANPFLQ SVHRCMTSRL LQLPDKHSVT ALLNTWAQSV
   781  HQACLSAA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MED15 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.56
Highest tissue expression: 52 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 52 nTPM
blood vessel: 43 nTPM
bone marrow: 40 nTPM
endometrium: 35 nTPM
skin: 34 nTPM
lung: 34 nTPM

Single-cell type

endometrial glandular cells: 163 nCPM
syncytiotrophoblasts: 132 nCPM
neutrophils: 131 nCPM
alveolar cells type 1: 129 nCPM
ocular epithelial cells: 127 nCPM
urothelial cells: 125 nCPM

Immune cell

T-reg: 29 nTPM
memory CD8 T-cell: 28 nTPM
gdT-cell: 24 nTPM
non-classical monocyte: 23 nTPM
NK-cell: 22 nTPM
memory CD4 T-cell: 17 nTPM

Brain region

cerebral cortex: 107 nTPM
white matter: 98 nTPM
cerebellum: 94 nTPM
pons: 88 nTPM
hypothalamus: 84 nTPM
hippocampal formation: 82 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MED15.

Disease | ImmuneIEDB

Conditions an epitope on MED15 was assayed in.

melanoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.24
gnomAD pLI: 1
gnomAD missense Z: 2.53
DepMap mean gene effect: -0.24
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

transcription coregulator activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

Coactivator CBP, KIX domain superfamily
Mediator of RNA polymerase II transcription subunit 15, N-terminal
ARC105/Med15 mediator subunit, central domain
ARC105/Med15 mediator subunit, C-terminal domain
ARC105/Med15 mediator subunit N-terminal KIX domain
ARC105/Med15 mediator subunit central domain
ARC105/Med15 mediator subunit C-terminal domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MED15 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MED15 as an antibody target. Whether an autoantibody or antibody against MED15 could matter depends on whether native MED15 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MED15 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MED15 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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