Seroatlas · Human Serome Atlas

MED22

Mediator of RNA polymerase II transcription subunit 22

Also known as: MED22_HUMAN, Med24, SRB6, SURF5

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q15528
Gene: MED22
Ensembl: ENSG00000148297
Chromosome: 9
Canonical length: 200 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013]

Canonical amino-acid sequenceUniProt

200 residues, UniProt reviewed canonical sequence.

>Q15528|MED22
     1  MAQQRALPQS KETLLQSYNK RLKDDIKSIM DNFTEIIKTA KIEDETQVSR ATQGEQDNYE
    61  MHVRAANIVR AGESLMKLVS DLKQFLILND FPSVNEAIDQ RNQQLRTLQE ECDRKLITLR
   121  DEISIDLYEL EEEYYSSSSS LCEANDLPLC EAYGRLDLDT DSADGLSAPL LASPEPSAGP
   181  LQVAAPAHSH AGGPGPTEHA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MED22 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.51
Highest tissue expression: 21 nTPM

Expression across tissuesHPA

Tissue

colon: 21 nTPM
endometrium: 21 nTPM
cerebellum: 20 nTPM
urinary bladder: 19 nTPM
cervix: 19 nTPM
spleen: 19 nTPM

Single-cell type

podocytes: 10 nCPM
microglia: 8.2 nCPM
proximal tubule cells: 7.4 nCPM
astrocytes: 7.3 nCPM
oligodendrocytes: 7.3 nCPM
renal connecting tubule cells: 7.1 nCPM

Immune cell

eosinophil: 2.6 nTPM
neutrophil: 2.3 nTPM
non-classical monocyte: 2.2 nTPM
classical monocyte: 1.5 nTPM
naive B-cell: 1.5 nTPM
plasmacytoid DC: 1.5 nTPM

Brain region

medulla oblongata: 14 nTPM
spinal cord: 12 nTPM
pons: 11 nTPM
cerebellum: 11 nTPM
midbrain: 11 nTPM
cerebral cortex: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.18
gnomAD pLI: 0
gnomAD missense Z: 0.37
DepMap mean gene effect: -1.29
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

transcription coregulator activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

Mediator of RNA polymerase II transcription subunit 22
Surfeit locus protein 5 subunit 22 of Mediator complex

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MED22 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MED22 as an antibody target. Whether an autoantibody or antibody against MED22 could matter depends on whether native MED22 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MED22 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MED22 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...