Seroatlas · Human Serome Atlas

MED25

Mediator of RNA polymerase II transcription subunit 25

Also known as: ACID1, ARC92, DKFZp434K0512, MED25_HUMAN, TCBAP0758

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q71SY5
Gene: MED25
Ensembl: ENSG00000104973
Chromosome: 19
Canonical length: 747 aa
Protein class: Disease related genes, Human disease related genes, Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2. [provided by RefSeq, Apr 2010]

Canonical amino-acid sequenceUniProt

747 residues, UniProt reviewed canonical sequence.

>Q71SY5|MED25
     1  MVPGSEGPAR AGSVVADVVF VIEGTANLGP YFEGLRKHYL LPAIEYFNGG PPAETDFGGD
    61  YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG IKFMGGGGES CSLIAEGLST
   121  ALQLFDDFKK MREQIGQTHR VCLLICNSPP YLLPAVESTT YSGCTTENLV QQIGERGIHF
   181  SIVSPRKLPA LRLLFEKAAP PALLEPLQPP TDVSQDPRHM VLVRGLVLPV GGGSAPGPLQ
   241  SKQPVPLPPA APSGATLSAA PQQPLPPVPP QYQVPGNLSA AQVAAQNAVE AAKNQKAGLG
   301  PRFSPITPLQ QAAPGVGPPF SQAPAPQLPP GPPGAPKPPP ASQPSLVSTV APGSGLAPTA
   361  QPGAPSMAGT VAPGGVSGPS PAQLGAPALG GQQSVSNKLL AWSGVLEWQE KPKPASVDAN
   421  TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL
   481  ESLKGLYRIM GNGFAGCVHF PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV
   541  ITNHKQVQQQ KLEQQQRGMG GQQAPPGLGP ILEDQARPSQ NLLQLRPPQP QPQGTVGASG
   601  ATGQPQPQGT AQPPPGAPQG PPGAASGPPP PGPILRPQNP GANPQLRSLL LNPPPPQTGV
   661  PPPQASLHHL QPPGAPALLP PPHQGLGQPQ LGPPLLHPPP AQSWPAQLPP RAPLPGQMLL
   721  SGGPRGPVPQ PGLQPSVMED DILMDLI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MED25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.49
Highest tissue expression: 80 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 80 nTPM
testis: 75 nTPM
adrenal gland: 69 nTPM
heart muscle: 42 nTPM
pituitary gland: 41 nTPM
ovary: 39 nTPM

Single-cell type

late spermatids: 185 nCPM
sertoli cells: 155 nCPM
erythrocyte progenitors: 116 nCPM
respiratory ciliated cells: 116 nCPM
esophageal apical cells: 91 nCPM
granulosa cells: 84 nCPM

Immune cell

neutrophil: 14 nTPM
eosinophil: 3.8 nTPM
naive B-cell: 3.3 nTPM
classical monocyte: 2.3 nTPM
memory B-cell: 2.3 nTPM
plasmacytoid DC: 2.2 nTPM

Brain region

choroid plexus: 70 nTPM
midbrain: 48 nTPM
cerebral cortex: 47 nTPM
medulla oblongata: 45 nTPM
hypothalamus: 45 nTPM
amygdala: 44 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MED25.

Disease | AllUniProt

Conditions MED25 is implicated in, by any mechanism.

Charcot-Marie-Tooth disease, axonal, type 2B2 (CMT2B2) MIM:605589
Basel-Vanagaite-Smirin-Yosef syndrome (BVSYS) MIM:616449

Disease | GeneticClinVar

8 pathogenic / likely-pathogenic of 838 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Congenital cataract-microcephaly-nevus flammeus simplex-severe intellectual disability syndrome
Charcot-Marie-Tooth disease type 2
Neurodevelopmental disorder
Inborn genetic diseases

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.65
gnomAD pLI: 0
gnomAD missense Z: 1.25
DepMap mean gene effect: -0.3
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Mediator complex, subunit Med25, PTOV domain
von Willebrand factor A-like domain superfamily
Mediator complex subunit 25, PTOV domain superfamily
Mediator complex subunit 25 PTOV activation and synapsin 2
Mediator complex, subunit Med25, synapsin 1
Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain
Mediator complex subunit 25 synapsin 1
Mediator complex subunit 25 von Willebrand factor type A

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MED25 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MED25 as an antibody target. Whether an autoantibody or antibody against MED25 could matter depends on whether native MED25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MED25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MED25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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