MED7
Mediator of RNA polymerase II transcription subunit 7
Also known as: CRSP33, CRSP9, MED7_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- O43513
- Gene
- MED7
- Ensembl
- ENSG00000155868
- Chromosome
- 5
- Canonical length
- 233 aa
- Protein class
- Predicted intracellular proteins
- Subcellular location
- Nuclear bodies
OverviewNCBI Gene
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
233 residues, UniProt reviewed canonical sequence.
>O43513|MED7
1 MGEPQQVSAL PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ CDDLIIRPLE
61 SQGIERLHPM QFDHKKELRK LNMSILINFL DLLDILIRSP GSIKREEKLE DLKLLFVHVH
121 HLINEYRPHQ ARETLRVMME VQKRQRLETA ERFQKHLERV IEMIQNCLAS LPDDLPHSEA
181 GMRVKTEPMD ADDSNNCTGQ NEHQRENSGH RRDQIIEKDA ALCVLIDEMN ERPLocalizationUniProt · AlphaFold · HPA
Whether an antibody against MED7 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.5
- Highest tissue expression
- 25 nTPM
Expression across tissuesHPA
Tissue
- testis: 25 nTPM
- choroid plexus: 14 nTPM
- kidney: 14 nTPM
- skeletal muscle: 13 nTPM
- epididymis: 13 nTPM
- spleen: 12 nTPM
Single-cell type
- early spermatids: 48 nCPM
- late spermatids: 31 nCPM
- late primary spermatocytes: 16 nCPM
- tuft cells: 12 nCPM
- mast cells: 9.6 nCPM
- t-cells: 7.7 nCPM
Immune cell
- basophil: 41 nTPM
- non-classical monocyte: 36 nTPM
- memory B-cell: 36 nTPM
- naive B-cell: 35 nTPM
- naive CD4 T-cell: 35 nTPM
- eosinophil: 31 nTPM
Brain region
- choroid plexus: 19 nTPM
- cerebellum: 18 nTPM
- white matter: 16 nTPM
- pons: 16 nTPM
- hypothalamus: 15 nTPM
- thalamus: 15 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.07
- gnomAD pLI
- 0.02
- gnomAD missense Z
- 1.17
- DepMap mean gene effect
- -0.93
- DepMap dependency class
- common
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- positive regulation of transcription elongation by RNA polymerase II
- positive regulation of transcription initiation by RNA polymerase II
- protein ubiquitination
- regulation of transcription by RNA polymerase II
- RNA polymerase II preinitiation complex assembly
- somatic stem cell population maintenance
- transcription initiation at RNA polymerase II promoter
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Mediator complex, subunit Med7/Med21-like
- Immune Modulating & Transcription Coactivator
- Mediator complex, subunit Med7
- Mediator complex, subunit Med7 superfmaily
- MED7 protein
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of MED7 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads MED7 as an antibody target. Whether an autoantibody or antibody against MED7 could matter depends on whether native MED7 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
MED7 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label MED7 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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