Seroatlas · Human Serome Atlas

RHOA

Transforming protein RhoA

Also known as: ARH12, ARHA, Rho12, RHOA_HUMAN, RHOH12

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P61586
Gene
RHOA
Ensembl
ENSG00000067560
Chromosome
3
Canonical length
193 aa
Protein class
Cancer-related genes, Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins, RAS pathway related proteins
Subcellular location
Plasma membrane,Cytosol

OverviewNCBI Gene

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Canonical amino-acid sequenceUniProt

193 residues, UniProt reviewed canonical sequence.

>P61586|RHOA
     1  MAAIRKKLVI VGDGACGKTC LLIVFSKDQF PEVYVPTVFE NYVADIEVDG KQVELALWDT
    61  AGQEDYDRLR PLSYPDTDVI LMCFSIDSPD SLENIPEKWT PEVKHFCPNV PIILVGNKKD
   121  LRNDEHTRRE LAKMKQEPVK PEEGRDMANR IGAFGYMECS AKTKDGVREV FEMATRAALQ
   181  ARRGKKKSGC LVL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RHOA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.29
Highest tissue expression
658 nTPM

Expression across tissuesHPA

Tissue

  • blood vessel: 658 nTPM
  • adipose tissue: 472 nTPM
  • bone marrow: 442 nTPM
  • tonsil: 424 nTPM
  • lung: 403 nTPM
  • breast: 390 nTPM

Single-cell type

  • microglia: 108 nCPM
  • cardiomyocytes: 99 nCPM
  • papillary tip epithelial cells: 95 nCPM
  • renal connecting tubule cells: 88 nCPM
  • renal collecting duct intercalated cells: 84 nCPM
  • podocytes: 78 nCPM

Immune cell

  • total PBMC: 1,778 nTPM
  • non-classical monocyte: 1,373 nTPM
  • intermediate monocyte: 1,316 nTPM
  • neutrophil: 1,264 nTPM
  • eosinophil: 1,108 nTPM
  • classical monocyte: 1,031 nTPM

Brain region

  • white matter: 320 nTPM
  • medulla oblongata: 318 nTPM
  • hypothalamus: 294 nTPM
  • thalamus: 267 nTPM
  • spinal cord: 264 nTPM
  • cerebellum: 256 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RHOA.

Disease | AllUniProt

Conditions RHOA is implicated in, by any mechanism.

  • Ectodermal dysplasia with facial dysmorphism and acral, ocular, and brain anomalies (EDFAOB) MIM:618727

Disease | GeneticClinVar

4 pathogenic / likely-pathogenic of 33 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.66
gnomAD pLI
0.6
gnomAD missense Z
3.36
DepMap mean gene effect
-0.39
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RHOA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RHOA as an antibody target. Whether an autoantibody or antibody against RHOA could matter depends on whether native RHOA is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RHOA is annotated at the cell surface, where native RHOA is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label RHOA as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RHOA. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...