Seroatlas · Human Serome Atlas

ADAM12

Disintegrin and metalloproteinase domain-containing protein 12

Also known as: ADA12_HUMAN, MCMPMltna, MLTN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O43184
Gene: ADAM12
Ensembl: ENSG00000148848
Chromosome: 10
Canonical length: 909 aa
Protein class: Predicted membrane proteins, Predicted secreted proteins, Transporters
Subcellular location: Plasma membrane
Secretome location: Secreted in female reproductive system

OverviewNCBI Gene

This gene encodes a member of a family of proteins that are structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Expression of this gene has been used as a maternal serum marker for pre-natal development. Alternative splicing results in multiple transcript variants encoding different isoforms. Shorter isoforms are secreted, while longer isoforms are membrane-bound form. [provided by RefSeq, Jan 2014]

Canonical amino-acid sequenceUniProt

909 residues, UniProt reviewed canonical sequence.

>O43184|ADAM12
     1  MAARPLPVSP ARALLLALAG ALLAPCEARG VSLWNQGRAD EVVSASVGSG DLWIPVKSFD
    61  SKNHPEVLNI RLQRESKELI INLERNEGLI ASSFTETHYL QDGTDVSLAR NYTVILGHCY
   121  YHGHVRGYSD SAVSLSTCSG LRGLIVFENE SYVLEPMKSA TNRYKLFPAK KLKSVRGSCG
   181  SHHNTPNLAA KNVFPPPSQT WARRHKRETL KATKYVELVI VADNREFQRQ GKDLEKVKQR
   241  LIEIANHVDK FYRPLNIRIV LVGVEVWNDM DKCSVSQDPF TSLHEFLDWR KMKLLPRKSH
   301  DNAQLVSGVY FQGTTIGMAP IMSMCTADQS GGIVMDHSDN PLGAAVTLAH ELGHNFGMNH
   361  DTLDRGCSCQ MAVEKGGCIM NASTGYPFPM VFSSCSRKDL ETSLEKGMGV CLFNLPEVRE
   421  SFGGQKCGNR FVEEGEECDC GEPEECMNRC CNATTCTLKP DAVCAHGLCC EDCQLKPAGT
   481  ACRDSSNSCD LPEFCTGASP HCPANVYLHD GHSCQDVDGY CYNGICQTHE QQCVTLWGPG
   541  AKPAPGICFE RVNSAGDPYG NCGKVSKSSF AKCEMRDAKC GKIQCQGGAS RPVIGTNAVS
   601  IETNIPLQQG GRILCRGTHV YLGDDMPDPG LVLAGTKCAD GKICLNRQCQ NISVFGVHEC
   661  AMQCHGRGVC NNRKNCHCEA HWAPPFCDKF GFGGSTDSGP IRQADNQGLT IGILVTILCL
   721  LAAGFVVYLK RKTLIRLLFT NKKTTIEKLR CVRPSRPPRG FQPCQAHLGH LGKGLMRKPP
   781  DSYPPKDNPR RLLQCQNVDI SRPLNGLNVP QPQSTQRVLP PLHRAPRAPS VPARPLPAKP
   841  ALRQAQGTCK PNPPQKPLPA DPLARTTRLT HALARTPGQW ETGLRLAPLR PAPQYPHQVP
   901  RSTHTAYIK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ADAM12 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.37
Highest tissue expression: 127 nTPM

Expression across tissuesHPA

Tissue

placenta: 127 nTPM
cervix: 12 nTPM
gallbladder: 8.7 nTPM
urinary bladder: 8.3 nTPM
adipose tissue: 7.5 nTPM
ovary: 6.4 nTPM

Single-cell type

extravillous trophoblasts: 1,570 nCPM
syncytiotrophoblasts: 617 nCPM
mast cells: 480 nCPM
adipocytes: 204 nCPM
leydig cells: 165 nCPM
oligodendrocyte progenitor cells: 135 nCPM

Immune cell

MAIT T-cell: 4.5 nTPM
T-reg: 0.9 nTPM
memory CD8 T-cell: 0.7 nTPM
gdT-cell: 0.2 nTPM
memory CD4 T-cell: 0.1 nTPM
total PBMC: 0.1 nTPM

Brain region

thalamus: 15 nTPM
medulla oblongata: 10 nTPM
choroid plexus: 8.4 nTPM
basal ganglia: 7.2 nTPM
pons: 6 nTPM
midbrain: 5.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.59
gnomAD pLI: 0
gnomAD missense Z: 1.27
DepMap mean gene effect: -0.07
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ADAM12 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ADAM12 as an antibody target. Whether an autoantibody or antibody against ADAM12 could matter depends on whether native ADAM12 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ADAM12 is annotated at the cell surface, where native ADAM12 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ADAM12 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...