Seroatlas · Human Serome Atlas

SLC9A5

Sodium/hydrogen exchanger 5

Also known as: NHE5, SL9A5_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q14940
Gene
SLC9A5
Ensembl
ENSG00000135740
Chromosome
16
Canonical length
896 aa
Protein class
Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins, Transporters
Subcellular location
Plasma membrane,Cytosol

OverviewNCBI Gene

Enables arrestin family protein binding activity and sodium:proton antiporter activity. Involved in regulation of intracellular pH and sodium ion transmembrane transport. Located in several cellular components, including focal adhesion; neuron spine; and recycling endosome membrane. Implicated in end stage renal disease. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

896 residues, UniProt reviewed canonical sequence.

>Q14940|SLC9A5
     1  MLRAALSLLA LPLAGAAEEP TQKPESPGEP PPGLELFRWQ WHEVEAPYLV ALWILVASLA
    61  KIVFHLSRKV TSLVPESCLL ILLGLVLGGI VLAVAKKAEY QLEPGTFFLF LLPPIVLDSG
   121  YFMPSRLFFD NLGAILTYAV VGTLWNAFTT GAALWGLQQA GLVAPRVQAG LLDFLLFGSL
   181  ISAVDPVAVL AVFEEVHVNE TLFIIVFGES LLNDAVTVVL YKVCNSFVEM GSANVQATDY
   241  LKGVASLFVV SLGGAAVGLV FAFLLALTTR FTKRVRIIEP LLVFLLAYAA YLTAEMASLS
   301  AILAVTMCGL GCKKYVEANI SHKSRTTVKY TMKTLASCAE TVIFMLLGIS AVDSSKWAWD
   361  SGLVLGTLIF ILFFRALGVV LQTWVLNQFR LVPLDKIDQV VMSYGGLRGA VAFALVILLD
   421  RTKVPAKDYF VATTIVVVFF TVIVQGLTIK PLVKWLKVKR SEHHKPTLNQ ELHEHTFDHI
   481  LAAVEDVVGH HGYHYWRDRW EQFDKKYLSQ LLMRRSAYRI RDQIWDVYYR LNIRDAISFV
   541  DQGGHVLSST GLTLPSMPSR NSVAETSVTN LLRESGSGAC LDLQVIDTVR SGRDREDAVM
   601  HHLLCGGLYK PRRRYKASCS RHFISEDAQE RQDKEVFQQN MKRRLESFKS TKHNICFTKS
   661  KPRPRKTGRR KKDGVANAEA TNGKHRGLGF QDTAAVILTV ESEEEEEESD SSETEKEDDE
   721  GIIFVARATS EVLQEGKVSG SLEVCPSPRI IPPSPTCAEK ELPWKSGQGD LAVYVSSETT
   781  KIVPVDMQTG WNQSISSLES LASPPCNQAP ILTCLPPHPR GTEEPQVPLH LPSDPRSSFA
   841  FPPSLAKAGR SRSESSADLP QQQELQPLMG HKDHTHLSPG TATSHWCIQF NRGSRL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SLC9A5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
12
Mean surface accessibility (rSASA)
0.43
Highest tissue expression
18 nTPM

Expression across tissuesHPA

Tissue

  • spleen: 18 nTPM
  • cerebellum: 15 nTPM
  • basal ganglia: 9.6 nTPM
  • cerebral cortex: 5 nTPM
  • hypothalamus: 4 nTPM
  • hippocampal formation: 3.6 nTPM

Single-cell type

  • cardiomyocytes: 70 nCPM
  • early primary spermatocytes: 44 nCPM
  • epicardial cells: 34 nCPM
  • retinal horizontal cells: 32 nCPM
  • retinal amacrine cells: 28 nCPM
  • brain inhibitory neurons: 24 nCPM

Immune cell

  • memory B-cell: 0.1 nTPM
  • memory CD8 T-cell: 0.1 nTPM
  • neutrophil: 0.1 nTPM
  • plasmacytoid DC: 0.1 nTPM
  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM

Brain region

  • basal ganglia: 11 nTPM
  • cerebral cortex: 10 nTPM
  • pons: 8.2 nTPM
  • cerebellum: 8 nTPM
  • hippocampal formation: 6.4 nTPM
  • amygdala: 5.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.69
gnomAD pLI
0
gnomAD missense Z
2.94
DepMap mean gene effect
0
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SLC9A5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SLC9A5 as an antibody target. Whether an autoantibody or antibody against SLC9A5 could matter depends on whether native SLC9A5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SLC9A5 is annotated at the cell surface, where native SLC9A5 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label SLC9A5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SLC9A5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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