POLR2K
DNA-directed RNA polymerases I, II, and III subunit RPABC4
Also known as: RPAB4_HUMAN, RPB10alpha, RPB12
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P53803
- Gene
- POLR2K
- Ensembl
- ENSG00000147669
- Chromosome
- 8
- Canonical length
- 58 aa
- Protein class
- Metabolic proteins, Predicted intracellular proteins, RNA polymerase related proteins
- Subcellular location
- Nucleoli fibrillar center
OverviewNCBI Gene
This gene encodes one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
58 residues, UniProt reviewed canonical sequence.
>P53803|POLR2K
1 MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK RLVVFDARLocalizationUniProt · AlphaFold · HPA
Whether an antibody against POLR2K can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.54
- Highest tissue expression
- 88 nTPM
Expression across tissuesHPA
Tissue
- cerebral cortex: 88 nTPM
- hypothalamus: 82 nTPM
- amygdala: 79 nTPM
- midbrain: 77 nTPM
- parathyroid gland: 76 nTPM
- hippocampal formation: 71 nTPM
Single-cell type
- parietal cells: 433 nCPM
- oocytes: 396 nCPM
- gastric progenitor cells: 345 nCPM
- extravillous trophoblasts: 248 nCPM
- esophageal apical cells: 233 nCPM
- hofbauer cells: 199 nCPM
Immune cell
- basophil: 73 nTPM
- T-reg: 71 nTPM
- plasmacytoid DC: 68 nTPM
- memory B-cell: 66 nTPM
- memory CD4 T-cell: 62 nTPM
- memory CD8 T-cell: 61 nTPM
Brain region
- hypothalamus: 45 nTPM
- cerebellum: 40 nTPM
- white matter: 40 nTPM
- pons: 38 nTPM
- spinal cord: 38 nTPM
- medulla oblongata: 38 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.88
- gnomAD pLI
- 0
- gnomAD missense Z
- 0.6
- DepMap mean gene effect
- -1.09
- DepMap dependency class
- common
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 17% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- regulation of transcription by RNA polymerase I
- transcription by RNA polymerase II
- transcription by RNA polymerase III
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
- RNA polymerase subunit RPABC4/transcription elongation factor Spt4
- RNA polymerase archaeal subunit P/eukaryotic subunit RPABC4
- DNA-directed RNA polymerases I, II, and III subunit RPABC4
- DNA directed RNA polymerase, 7 kDa subunit
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of POLR2K in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads POLR2K as an antibody target. Whether an autoantibody or antibody against POLR2K could matter depends on whether native POLR2K is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
POLR2K is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label POLR2K as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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