Seroatlas · Human Serome Atlas

POLR3D

DNA-directed RNA polymerase III subunit RPC4

Also known as: BN51T, C53, RPC4, RPC4_HUMAN, TSBN51

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P05423
Gene: POLR3D
Ensembl: ENSG00000168495
Chromosome: 8
Canonical length: 398 aa
Protein class: Metabolic proteins, Predicted intracellular proteins, RNA polymerase related proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

This gene complements a temperature-sensitive mutant isolated from the BHK-21 Syrian hamster cell line. It leads to a block in progression through the G1 phase of the cell cycle at nonpermissive temperatures. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

398 residues, UniProt reviewed canonical sequence.

>P05423|POLR3D
     1  MSEGNAAGEP STPGGPRPLL TGARGLIGRR PAPPLTPGRL PSIRSRDLTL GGVKKKTFTP
    61  NIISRKIKEE PKEEVTVKKE KRERDRDRQR EGHGRGRGRP EVIQSHSIFE QGPAEMMKKK
   121  GNWDKTVDVS DMGPSHIINI KKEKRETDEE TKQILRMLEK DDFLDDPGLR NDTRNMPVQL
   181  PLAHSGWLFK EENDEPDVKP WLAGPKEEDM EVDIPAVKVK EEPRDEEEEA KMKAPPKAAR
   241  KTPGLPKDVS VAELLRELSL TKEEELLFLQ LPDTLPGQPP TQDIKPIKTE VQGEDGQVVL
   301  IKQEKDREAK LAENACTLAD LTEGQVGKLL IRKSGRVQLL LGKVTLDVTM GTACSFLQEL
   361  VSVGLGDSRT GEMTVLGHVK HKLVCSPDFE SLLDHKHR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POLR3D can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.61
Highest tissue expression: 12 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 12 nTPM
tongue: 7.7 nTPM
pancreas: 6.5 nTPM
ovary: 6.2 nTPM
placenta: 6.2 nTPM
adipose tissue: 6.1 nTPM

Single-cell type

late primary spermatocytes: 74 nCPM
differentiating spermatogonia: 55 nCPM
late spermatids: 53 nCPM
extravillous trophoblasts: 43 nCPM
early primary spermatocytes: 40 nCPM
early spermatids: 40 nCPM

Immune cell

plasmacytoid DC: 3.5 nTPM
naive CD4 T-cell: 2.9 nTPM
NK-cell: 2.9 nTPM
memory CD8 T-cell: 2.6 nTPM
MAIT T-cell: 2.5 nTPM
memory B-cell: 2.4 nTPM

Brain region

thalamus: 14 nTPM
white matter: 14 nTPM
choroid plexus: 14 nTPM
cerebral cortex: 14 nTPM
medulla oblongata: 14 nTPM
midbrain: 14 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.25
gnomAD pLI: 0
gnomAD missense Z: 0.36
DepMap mean gene effect: -0.54
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

DNA-directed RNA polymerase III subunit RPC4
RNA polymerase III RPC4

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of POLR3D in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POLR3D as an antibody target. Whether an autoantibody or antibody against POLR3D could matter depends on whether native POLR3D is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POLR3D is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POLR3D as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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