Seroatlas · Human Serome Atlas

POLR3H

DNA-directed RNA polymerase III subunit RPC8

Also known as: C25, KIAA1665, RPC8, RPC8_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y535
Gene: POLR3H
Ensembl: ENSG00000100413
Chromosome: 22
Canonical length: 204 aa
Protein class: Metabolic proteins, Predicted intracellular proteins, RNA polymerase related proteins
Subcellular location: Nucleoplasm,Vesicles,Centrosome

OverviewNCBI Gene

Enables DNA-directed RNA polymerase activity. Involved in transcription by RNA polymerase III. Located in centrosome and nucleoplasm. Part of RNA polymerase III complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

204 residues, UniProt reviewed canonical sequence.

>Q9Y535|POLR3H
     1  MFVLVEMVDT VRIPPWQFER KLNDSIAEEL NKKLANKVVY NVGLCICLFD ITKLEDAYVF
    61  PGDGASHTKV HFRCVVFHPF LDEILIGKIK GCSPEGVHVS LGFFDDILIP PESLQQPAKF
   121  DEAEQVWVWE YETEEGAHDL YMDTGEEIRF RVVDESFVDT SPTGPSSADA TTSSEELPKK
   181  EAPYTLVGSI SEPGLGLLSW WTSN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POLR3H can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.34
Highest tissue expression: 42 nTPM

Expression across tissuesHPA

Tissue

cerebral cortex: 42 nTPM
amygdala: 41 nTPM
basal ganglia: 39 nTPM
hippocampal formation: 32 nTPM
midbrain: 31 nTPM
hypothalamus: 27 nTPM

Single-cell type

late spermatids: 301 nCPM
cardiomyocytes: 169 nCPM
oocytes: 111 nCPM
differentiating spermatogonia: 79 nCPM
epicardial cells: 71 nCPM
esophageal suprabasal cells: 63 nCPM

Immune cell

non-classical monocyte: 19 nTPM
intermediate monocyte: 18 nTPM
memory B-cell: 17 nTPM
myeloid DC: 14 nTPM
naive B-cell: 14 nTPM
plasmacytoid DC: 13 nTPM

Brain region

midbrain: 35 nTPM
thalamus: 34 nTPM
medulla oblongata: 33 nTPM
amygdala: 31 nTPM
spinal cord: 31 nTPM
hippocampal formation: 29 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.03
gnomAD pLI: 0.01
gnomAD missense Z: 0.14
DepMap mean gene effect: -1.59
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

RNA polymerase Rpb7-like , N-terminal
Nucleic acid-binding, OB-fold
RNA polymerase Rpb7-like, N-terminal domain superfamily
RNA polymerase subunit Rpb7-like
SHS2 domain found in N terminus of Rpb7p/Rpc25p/MJ0397
DNA-directed RNA polymerase, subunit E/RPC8
RNA polymerase III, subunit Rpc25
RNA polymerase III subunit Rpc25

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of POLR3H in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POLR3H as an antibody target. Whether an autoantibody or antibody against POLR3H could matter depends on whether native POLR3H is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POLR3H is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POLR3H as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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