POLR2J
DNA-directed RNA polymerase II subunit RPB11-a
Also known as: hRPB14, POLR2J1, RPB11, RPB11_HUMAN, RPB11A, RPB11m
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P52435
- Gene
- POLR2J
- Ensembl
- ENSG00000005075
- Chromosome
- 7
- Canonical length
- 117 aa
- Protein class
- Metabolic proteins, Predicted intracellular proteins, RNA polymerase related proteins
- Subcellular location
- Nucleoplasm
OverviewNCBI Gene
This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
117 residues, UniProt reviewed canonical sequence.
>P52435|POLR2J
1 MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL LKDPQVLFAG
61 YKVPHPLEHK IIIRVQTTPD YSPQEAFTNA ITDLISELSL LEERFRVAIK DKQEGIELocalizationUniProt · AlphaFold · HPA
Whether an antibody against POLR2J can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.37
- Highest tissue expression
- 187 nTPM
Expression across tissuesHPA
Tissue
- skeletal muscle: 187 nTPM
- tongue: 119 nTPM
- heart muscle: 118 nTPM
- liver: 93 nTPM
- amygdala: 90 nTPM
- midbrain: 89 nTPM
Single-cell type
- oocytes: 636 nCPM
- esophageal apical cells: 402 nCPM
- epididymal principal cells: 285 nCPM
- esophageal suprabasal cells: 279 nCPM
- hofbauer cells: 258 nCPM
- differentiating spermatogonia: 249 nCPM
Immune cell
- eosinophil: 197 nTPM
- basophil: 182 nTPM
- neutrophil: 176 nTPM
- T-reg: 139 nTPM
- memory B-cell: 134 nTPM
- total PBMC: 125 nTPM
Brain region
- thalamus: 46 nTPM
- hypothalamus: 45 nTPM
- medulla oblongata: 43 nTPM
- spinal cord: 42 nTPM
- cerebellum: 41 nTPM
- cerebral cortex: 40 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.37
- gnomAD pLI
- 0.01
- gnomAD missense Z
- 1.3
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of POLR2J in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads POLR2J as an antibody target. Whether an autoantibody or antibody against POLR2J could matter depends on whether native POLR2J is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
POLR2J is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label POLR2J as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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