Seroatlas · Human Serome Atlas

POLR3A

DNA-directed RNA polymerase III subunit RPC1

Also known as: C160, hRPC155, RPC1, RPC1_HUMAN, RPC155

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O14802
Gene
POLR3A
Ensembl
ENSG00000148606
Chromosome
10
Canonical length
1390 aa
Protein class
Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Plasma proteins, Potential drug targets, Predicted intracellular proteins, RNA polymerase related proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

The protein encoded by this gene is the catalytic component of RNA polymerase III, which synthesizes small RNAs. The encoded protein also acts as a sensor to detect foreign DNA and trigger an innate immune response. [provided by RefSeq, Aug 2011]

Canonical amino-acid sequenceUniProt

1390 residues, UniProt reviewed canonical sequence.

>O14802|POLR3A
     1  MVKEQFRETD VAKKISHICF GMKSPEEMRQ QAHIQVVSKN LYSQDNQHAP LLYGVLDHRM
    61  GTSEKDRPCE TCGKNLADCL GHYGYIDLEL PCFHVGYFRA VIGILQMICK TCCHIMLSQE
   121  EKKQFLDYLK RPGLTYLQKR GLKKKISDKC RKKNICHHCG AFNGTVKKCG LLKIIHEKYK
   181  TNKKVVDPIV SNFLQSFETA IEHNKEVEPL LGRAQENLNP LVVLNLFKRI PAEDVPLLLM
   241  NPEAGKPSDL ILTRLLVPPL CIRPSVVSDL KSGTNEDDLT MKLTEIIFLN DVIKKHRISG
   301  AKTQMIMEDW DFLQLQCALY INSELSGIPL NMAPKKWTRG FVQRLKGKQG RFRGNLSGKR
   361  VDFSGRTVIS PDPNLRIDEV AVPVHVAKIL TFPEKVNKAN INFLRKLVQN GPEVHPGANF
   421  IQQRHTQMKR FLKYGNREKM AQELKYGDIV ERHLIDGDVV LFNRQPSLHK LSIMAHLARV
   481  KPHRTFRFNE CVCTPYNADF DGDEMNLHLP QTEEAKAEAL VLMGTKANLV TPRNGEPLIA
   541  AIQDFLTGAY LLTLKDTFFD RAKACQIIAS ILVGKDEKIK VRLPPPTILK PVTLWTGKQI
   601  FSVILRPSDD NPVRANLRTK GKQYCGKGED LCANDSYVTI QNSELMSGSM DKGTLGSGSK
   661  NNIFYILLRD WGQNEAADAM SRLARLAPVY LSNRGFSIGI GDVTPGQGLL KAKYELLNAG
   721  YKKCDEYIEA LNTGKLQQQP GCTAEETLEA LILKELSVIR DHAGSACLRE LDKSNSPLTM
   781  ALCGSKGSFI NISQMIACVG QQAISGSRVP DGFENRSLPH FEKHSKLPAA KGFVANSFYS
   841  GLTPTEFFFH TMAGREGLVD TAVKTAETGY MQRRLVKSLE DLCSQYDLTV RSSTGDIIQF
   901  IYGGDGLDPA AMEGKDEPLE FKRVLDNIKA VFPCPSEPAL SKNELILTTE SIMKKSEFLC
   961  CQDSFLQEIK KFIKGVSEKI KKTRDKYGIN DNGTTEPRVL YQLDRITPTQ VEKFLETCRD
  1021  KYMRAQMEPG SAVGALCAQS IGEPGTQMTL KTFHFAGVAS MNITLGVPRI KEIINASKAI
  1081  STPIITAQLD KDDDADYARL VKGRIEKTLL GEISEYIEEV FLPDDCFILV KLSLERIRLL
  1141  RLEVNAETVR YSICTSKLRV KPGDVAVHGE AVVCVTPREN SKSSMYYVLQ FLKEDLPKVV
  1201  VQGIPEVSRA VIHIDEQSGK EKYKLLVEGD NLRAVMATHG VKGTRTTSNN TYEVEKTLGI
  1261  EAARTTIINE IQYTMVNHGM SIDRRHVMLL SDLMTYKGEV LGITRFGLAK MKESVLMLAS
  1321  FEKTADHLFD AAYFGQKDSV CGVSECIIMG IPMNIGTGLF KLLHKADRDP NPPKRPLIFD
  1381  TNEFHIPLVT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POLR3A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.28
Highest tissue expression
11 nTPM

Expression across tissuesHPA

Tissue

  • cerebellum: 11 nTPM
  • skeletal muscle: 11 nTPM
  • pituitary gland: 9.7 nTPM
  • cerebral cortex: 8.7 nTPM
  • tongue: 6.3 nTPM
  • retina: 5.9 nTPM

Single-cell type

  • corticotrophs: 130 nCPM
  • somatotrophs: 124 nCPM
  • cone photoreceptor cells: 66 nCPM
  • myonuclei: 58 nCPM
  • retinal horizontal cells: 58 nCPM
  • adrenal cortex cells: 53 nCPM

Immune cell

  • memory CD8 T-cell: 2.1 nTPM
  • intermediate monocyte: 1.9 nTPM
  • naive CD8 T-cell: 1.9 nTPM
  • gdT-cell: 1.8 nTPM
  • T-reg: 1.7 nTPM
  • MAIT T-cell: 1.6 nTPM

Brain region

  • cerebral cortex: 7.2 nTPM
  • pons: 5.5 nTPM
  • basal ganglia: 5.2 nTPM
  • thalamus: 3.9 nTPM
  • medulla oblongata: 3.8 nTPM
  • white matter: 3.5 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about POLR3A.

Disease | AllUniProt

Conditions POLR3A is implicated in, by any mechanism.

  • Leukodystrophy, hypomyelinating, 7, with or without oligodontia and/or hypogonadotropic hypogonadism (HLD7) MIM:607694
  • Wiedemann-Rautenstrauch syndrome (WDRTS) MIM:264090

Disease | GeneticClinVar

132 pathogenic / likely-pathogenic of 1,353 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Leukodystrophy, hypomyelinating, 7, with or without oligodontia and/or hypogonadotropic hypogonadism
  • Neonatal pseudo-hydrocephalic progeroid syndrome
  • Leukodystrophy
  • POLR3A-related disorder
  • POLR-related leukodystrophy

Disease | ImmuneIEDB

Conditions an epitope on POLR3A was assayed in.

Disease | AutoantibodyPubMed

Conditions in which antibodies against POLR3A are reported. Each links to that disease's full target list.

Showing 12 of 15 — disease pages carrying at least 10 antigens.

ReferencesPubMed · IEDB

Publications for POLR3A from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

122 publications

Show 20 more of 122 total

Reference: B cellIEDB

1 publication

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.88
gnomAD pLI
0
gnomAD missense Z
2.31
DepMap mean gene effect
-2.31
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of POLR3A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POLR3A as an antibody target. Whether an autoantibody or antibody against POLR3A could matter depends on whether native POLR3A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POLR3A is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POLR3A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/POLR3A. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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