Seroatlas · Human Serome Atlas

RPAP3

RNA polymerase II-associated protein 3

Also known as: FLJ21908, hSpagh, RPAP3_HUMAN, spag, Tah1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H6T3
Gene: RPAP3
Ensembl: ENSG00000005175
Chromosome: 12
Canonical length: 665 aa
Protein class: Predicted intracellular proteins
Subcellular location: Primary cilium,Primary cilium tip,Basal body,Cytosol,Acrosome

OverviewNCBI Gene

This gene encodes an RNA polymerase II-associated protein. The encoded protein may function in transcriptional regulation and may also regulate apoptosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]

Canonical amino-acid sequenceUniProt

665 residues, UniProt reviewed canonical sequence.

>Q9H6T3|RPAP3
     1  MTSANKAIEL QLQVKQNAEE LQDFMRDLEN WEKDIKQKDM ELRRQNGVPE ENLPPIRNGN
    61  FRKKKKGKAK ESSKKTREEN TKNRIKSYDY EAWAKLDVDR ILDELDKDDS THESLSQESE
   121  SEEDGIHVDS QKALVLKEKG NKYFKQGKYD EAIDCYTKGM DADPYNPVLP TNRASAYFRL
   181  KKFAVAESDC NLAVALNRSY TKAYSRRGAA RFALQKLEEA KKDYERVLEL EPNNFEATNE
   241  LRKISQALAS KENSYPKEAD IVIKSTEGER KQIEAQQNKQ QAISEKDRGN GFFKEGKYER
   301  AIECYTRGIA ADGANALLPA NRAMAYLKIQ KYEEAEKDCT QAILLDGSYS KAFARRGTAR
   361  TFLGKLNEAK QDFETVLLLE PGNKQAVTEL SKIKKELIEK GHWDDVFLDS TQRQNVVKPI
   421  DNPPHPGSTK PLKKVIIEET GNLIQTIDVP DSTTAAAPEN NPINLANVIA ATGTTSKKNS
   481  SQDDLFPTSD TPRAKVLKIE EVSDTSSLQP QASLKQDVCQ SYSEKMPIEI EQKPAQFATT
   541  VLPPIPANSF QLESDFRQLK SSPDMLYQYL KQIEPSLYPK LFQKNLDPDV FNQIVKILHD
   601  FYIEKEKPLL IFEILQRLSE LKRFDMAVMF MSETEKKIAR ALFNHIDKSG LKDSSVEELK
   661  KRYGG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPAP3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.43
Highest tissue expression: 28 nTPM

Expression across tissuesHPA

Tissue

lymph node: 28 nTPM
tonsil: 27 nTPM
tongue: 22 nTPM
thymus: 22 nTPM
skeletal muscle: 22 nTPM
appendix: 18 nTPM

Single-cell type

fallopian tube ciliated cells: 71 nCPM
oocytes: 65 nCPM
endometrial ciliated cells: 61 nCPM
epididymal efferent duct ciliated cells: 56 nCPM
early primary spermatocytes: 53 nCPM
epididymal basal cells: 51 nCPM

Immune cell

eosinophil: 33 nTPM
non-classical monocyte: 26 nTPM
memory B-cell: 21 nTPM
intermediate monocyte: 21 nTPM
neutrophil: 20 nTPM
naive B-cell: 18 nTPM

Brain region

midbrain: 20 nTPM
cerebellum: 20 nTPM
hypothalamus: 18 nTPM
pons: 18 nTPM
white matter: 18 nTPM
cerebral cortex: 17 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.58
gnomAD pLI: 0
gnomAD missense Z: 0.43
DepMap mean gene effect: -0.81
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

protein stabilization

Cellular components

Protein domainsUniProt · Pfam · InterPro

Tetratricopeptide-like helical domain superfamily
Tetratricopeptide repeat
RNA-polymerase II-associated protein 3-like, C-terminal domain
Tetratricopeptide repeat
Tetratricopeptide repeat
Tetratricopeptide repeat
Potential Monad-binding region of RPAP3
RNA polymerase II-associated protein 3

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RPAP3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPAP3 as an antibody target. Whether an autoantibody or antibody against RPAP3 could matter depends on whether native RPAP3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPAP3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPAP3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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