Seroatlas · Human Serome Atlas

SUPT5H

Transcription elongation factor SPT5

Also known as: FLJ34157, SPT5, SPT5H, SPT5H_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O00267
Gene: SUPT5H
Ensembl: ENSG00000196235
Chromosome: 19
Canonical length: 1087 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Enables enzyme binding activity and protein heterodimerization activity. Involved in positive regulation of macroautophagy; regulation of DNA-templated transcription; and transcription elongation by RNA polymerase II. Located in nucleoplasm. Part of DSIF complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1087 residues, UniProt reviewed canonical sequence.

>O00267|SUPT5H
     1  MSDSEDSNFS EEEDSERSSD GEEAEVDEER RSAAGSEKEE EPEDEEEEEE EEEYDEEEEE
    61  EDDDRPPKKP RHGGFILDEA DVDDEYEDED QWEDGAEDIL EKEEIEASNI DNVVLDEDRS
   121  GARRLQNLWR DQREEELGEY YMKKYAKSSV GETVYGGSDE LSDDITQQQL LPGVKDPNLW
   181  TVKCKIGEER ATAISLMRKF IAYQFTDTPL QIKSVVAPEH VKGYIYVEAY KQTHVKQAIE
   241  GVGNLRLGYW NQQMVPIKEM TDVLKVVKEV ANLKPKSWVR LKRGIYKDDI AQVDYVEPSQ
   301  NTISLKMIPR IDYDRIKARM SLKDWFAKRK KFKRPPQRLF DAEKIRSLGG DVASDGDFLI
   361  FEGNRYSRKG FLFKSFAMSA VITEGVKPTL SELEKFEDQP EGIDLEVVTE STGKEREHNF
   421  QPGDNVEVCE GELINLQGKI LSVDGNKITI MPKHEDLKDM LEFPAQELRK YFKMGDHVKV
   481  IAGRFEGDTG LIVRVEENFV ILFSDLTMHE LKVLPRDLQL CSETASGVDV GGQHEWGELV
   541  QLDPQTVGVI VRLERETFQV LNMYGKVVTV RHQAVTRKKD NRFAVALDSE QNNIHVKDIV
   601  KVIDGPHSGR EGEIRHLFRS FAFLHCKKLV ENGGMFVCKT RHLVLAGGSK PRDVTNFTVG
   661  GFAPMSPRIS SPMHPSAGGQ RGGFGSPGGG SGGMSRGRGR RDNELIGQTV RISQGPYKGY
   721  IGVVKDATES TARVELHSTC QTISVDRQRL TTVGSRRPGG MTSTYGRTPM YGSQTPMYGS
   781  GSRTPMYGSQ TPLQDGSRTP HYGSQTPLHD GSRTPAQSGA WDPNNPNTPS RAEEEYEYAF
   841  DDEPTPSPQA YGGTPNPQTP GYPDPSSPQV NPQYNPQTPG TPAMYNTDQF SPYAAPSPQG
   901  SYQPSPSPQS YHQVAPSPAG YQNTHSPASY HPTPSPMAYQ ASPSPSPVGY SPMTPGAPSP
   961  GGYNPHTPGS GIEQNSSDWV TTDIQVKVRD TYLDTQVVGQ TGVIRSVTGG MCSVYLKDSE
  1021  KVVSISSEHL EPITPTKNNK VKVILGEDRE ATGVLLSIDG EDGIVRMDLD EQLKILNLRF
  1081  LGKLLEA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SUPT5H can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.47
Highest tissue expression: 100 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 100 nTPM
testis: 97 nTPM
skeletal muscle: 82 nTPM
pituitary gland: 69 nTPM
cerebellum: 66 nTPM
blood vessel: 62 nTPM

Single-cell type

endometrial glandular cells: 148 nCPM
neutrophils: 139 nCPM
pdcs: 136 nCPM
salivary acinar cells: 123 nCPM
late spermatids: 122 nCPM
undifferentiated spermatogonia: 121 nCPM

Immune cell

basophil: 49 nTPM
neutrophil: 47 nTPM
plasmacytoid DC: 43 nTPM
memory B-cell: 23 nTPM
classical monocyte: 22 nTPM
eosinophil: 19 nTPM

Brain region

cerebral cortex: 83 nTPM
white matter: 75 nTPM
basal ganglia: 72 nTPM
thalamus: 72 nTPM
amygdala: 69 nTPM
hypothalamus: 64 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about SUPT5H.

Disease | ImmuneIEDB

Conditions an epitope on SUPT5H was assayed in.

abacavir allergy T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.07
gnomAD pLI: 1
gnomAD missense Z: 4.56
DepMap mean gene effect: -1.43
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 11% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KOW
Translation protein SH3-like domain superfamily
Large ribosomal subunit protein uL2, domain 2
Spt5 C-terminal domain
KOW motif
NGN domain
NusG-like, N-terminal
Transcription elongation factor Spt5, eukaryote
Spt5 transcription elongation factor, N-terminal
NusG, N-terminal domain superfamily
NGN domain, eukaryotic
Transcription elongation factor SPT5
Spt5, KOW domain repeat 1
Spt5, KOW domain repeat 2
Spt5, KOW domain repeat 3
Spt5, KOW domain repeat 4
Spt5, KOW domain repeat 5
Spt5, KOW domain repeat 6, metazoa
Spt5, KOW domain repeat 7
Spt5, KOWx domain
Early transcription elongation factor of RNA pol II, NGN section
Spt5 transcription elongation factor, acidic N-terminal
Transcription elongation factor SPT5, KOWx domain
Transcription elongation factor SPT5, KOW1 domain
Transcription elongation factor SPT5, second KOW domain
Transcription elongation factor SPT5, seventh KOW domain
Transcription elongation factor SPT5, sixth KOW domain
Transcription elongation factor SPT5, fifth KOW domain
Transcription elongation factor SPT5, fourth KOW domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SUPT5H in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SUPT5H as an antibody target. Whether an autoantibody or antibody against SUPT5H could matter depends on whether native SUPT5H is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SUPT5H is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SUPT5H as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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