Seroatlas · Human Serome Atlas

POLR3F

DNA-directed RNA polymerase III subunit RPC6

Also known as: C34, RPC39, RPC6, RPC6_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H1D9
Gene: POLR3F
Ensembl: ENSG00000132664
Chromosome: 20
Canonical length: 316 aa
Protein class: Disease related genes, Metabolic proteins, Predicted intracellular proteins, RNA polymerase related proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Canonical amino-acid sequenceUniProt

316 residues, UniProt reviewed canonical sequence.

>Q9H1D9|POLR3F
     1  MAEVKVKVQP PDADPVEIEN RIIELCHQFP HGITDQVIQN EMPHIEAQQR AVAINRLLSM
    61  GQLDLLRSNT GLLYRIKDSQ NAGKMKGSDN QEKLVYQIIE DAGNKGIWSR DIRYKSNLPL
   121  TEINKILKNL ESKKLIKAVK SVAASKKKVY MLYNLQPDRS VTGGAWYSDQ DFESEFVEVL
   181  NQQCFKFLQS KAETARESKQ NPMIQRNSSF ASSHEVWKYI CELGISKVEL SMEDIETILN
   241  TLIYDGKVEM TIIAAKEGTV GSVDGHMKLY RAVNPIIPPT GLVRAPCGLC PVFDDCHEGG
   301  EISPSNCIYM TEWLEF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POLR3F can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.37
Highest tissue expression: 25 nTPM

Expression across tissuesHPA

Tissue

cerebellum: 25 nTPM
retina: 14 nTPM
cerebral cortex: 12 nTPM
hypothalamus: 9.9 nTPM
thyroid gland: 9.9 nTPM
testis: 9.5 nTPM

Single-cell type

late primary spermatocytes: 63 nCPM
early primary spermatocytes: 35 nCPM
differentiating spermatogonia: 30 nCPM
oocytes: 28 nCPM
brain excitatory neurons: 26 nCPM
bergmann glia: 25 nCPM

Immune cell

MAIT T-cell: 5.5 nTPM
NK-cell: 5 nTPM
myeloid DC: 4.6 nTPM
T-reg: 4.6 nTPM
intermediate monocyte: 4.5 nTPM
memory CD4 T-cell: 4.5 nTPM

Brain region

cerebellum: 21 nTPM
white matter: 21 nTPM
hypothalamus: 20 nTPM
cerebral cortex: 19 nTPM
hippocampal formation: 17 nTPM
pons: 16 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about POLR3F.

Disease | AllUniProt

Conditions POLR3F is implicated in, by any mechanism.

Immunodeficiency 101, varicella zoster virus-specific (IMD101) MIM:619872

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 184 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Immunodeficiency 101 (varicella zoster virus-specific)

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.07
gnomAD pLI: 0
gnomAD missense Z: 1.65
DepMap mean gene effect: -1.32
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 13% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Winged helix-like DNA-binding domain superfamily
Winged helix DNA-binding domain superfamily
RNA polymerase Rpc34
RNA polymerase Rpc34-like
RNA polymerase Rpc34 subunit

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of POLR3F in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POLR3F as an antibody target. Whether an autoantibody or antibody against POLR3F could matter depends on whether native POLR3F is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POLR3F is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POLR3F as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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